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J. Bacteriol., 06 1995, 3613-3615, Vol 177, No. 12
Copyright © 1995, American Society for Microbiology

Ferrochelatase activity and protoporphyrin IX utilization in Haemophilus influenzae

MR Loeb
University of Rochester Medical Center, New York 14642, USA.

Previous research showed that the heme-requiring human pathogen Haemophilus influenzae lacks the first six of the seven enzymes required for heme synthesis, starting with the precursor, 5-amino levulinic acid. In this study, I demonstrated either directly or by reasonable inference that all 57 strains of H. influenzae examined, including 2 unable to grow on protoporphyrin IX, possess ferrochelatase, which catalyzes heme formation by insertion of Fe2+ into the protoporphyrin IX nucleus and which is the last enzyme in the heme synthetic pathway. Further, I showed that this enzyme can also function in the reverse direction, releasing Fe2+ from heme.


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