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 Previous Article

J. Bacteriol., 06 1995, 3619-3622, Vol 177, No. 12
Copyright © 1995, American Society for Microbiology

Topoisomerase activity during the heat shock response in Escherichia coli K-12

R Camacho-Carranza, J Membrillo-Hernandez, J Ramirez-Santos, J Castro- Dorantes, V Chagoya de Sanchez and MC Gomez-Eichelmann
Departamento de Biologia Molecular, Universidad Nacional Autonoma de Mexico, Mexico, D.F.

During the upshift of temperature from 30 to 42, 45, 47, or 50 degrees C, an increase in the level of supercoiling of a reporter plasmid was observed. This increase was present in groE and dnaK mutants but was inhibited in cells treated with chloramphenicol and novobiocin. The intracellular [ATP]/[ADP] ratio increased rapidly after an upshift in temperature from 30 to 47 degrees C and then decreased to reach a level above that observed at 30 degrees C. These results suggest that gyrase and proteins synthesized during heat shock are responsible for the changes seen in plasmid supercoiling. Proteins GroE and DnaK are probably not involved in this phenomenon.


This article has been cited by other articles:

  • Reyes-Dominguez, Y., Contreras-Ferrat, G., Ramirez-Santos, J., Membrillo-Hernandez, J., Gomez-Eichelmann, M. C. (2003). Plasmid DNA Supercoiling and Gyrase Activity in Escherichia coli Wild-Type and rpoS Stationary-Phase Cells. J. Bacteriol. 185: 1097-1100 [Abstract] [Full Text]  
  • Yigit, H., Reznikoff, W. S. (1998). Escherichia coli DNA Topoisomerase I and Suppression of Killing by Tn5 Transposase Overproduction: Topoisomerase I Modulates Tn5 Transposition. J. Bacteriol. 180: 5866-5874 [Abstract] [Full Text]  


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