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J. Bacteriol., Jul 1995, 4166-4170, Vol 177, No. 14
Copyright © 1995, American Society for Microbiology

Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance

R Munoz, M Bustamante and AG de la Campa
Unidad de Genetica Bacteriana, Instituto de Salud Carlos III, Majadahonda, Spain.

We report the cloning of the gyrB gene from Streptococcus pneumoniae 533 that carries the nov-1 allele. The gyrB gene codes for a protein homologous to the gyrase B subunit of archaebacteria and eubacteria. The same amino acid substitution (Ser-127 to Leu) confers novobiocin resistance on four isolates of S. pneumoniae. This amino acid position is equivalent to Val-120 of Escherichia coli GyrB, a residue that lies inside the ATP-binding domain as revealed by the crystal structure of the protein.


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