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J. Bacteriol., Jul 1995, 4176-4178, Vol 177, No. 14
Copyright © 1995, American Society for Microbiology

Regulation of the multiple antibiotic resistance (mar) regulon by marORA sequences in Escherichia coli

RG Martin, PS Nyantakyi and JL Rosner
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892-0560, USA.

The mar operon and adjacent sequences were subcloned on a low-copy- number plasmid to identify essential regulatory elements. A 1.1-kbp fragment containing 133 bp of the operator-promoter region (marO), the full marRA gene sequences, and only 10 of 72 marB codons provided a dela mar strain with normal repressibility and inducibility and the ability to beget mar constitutive mutants.


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