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J. Bacteriol., Jul 1995, 4183-4186, Vol 177, No. 14
Copyright © 1995, American Society for Microbiology

Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae

S Sirois and G Szatmari
Departement de Microbiologie et Immunologie, Universite de Montreal, Quebec, Canada.

XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to cloned E. coli xerC and xerD genes and a cer-nmr plasmid-based resolution assay, the presence of these genes in several species of Enterobacteriaceae is shown.

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