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J. Bacteriol., 07 1995, 4187-4189, Vol 177, No. 14
Copyright © 1995, American Society for Microbiology

Cloning and primary structure of the chiA gene from Aeromonas caviae

Y Sitrit, CE Vorgias, I Chet and AB Oppenheim
Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel.

The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.

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