This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomme, P.
Right arrow Articles by Kilburn, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomme, P.
Right arrow Articles by Kilburn, D. G.

 Previous Article  |  Next Article 

J. Bacteriol., 08 1995, 4356-4363, Vol 177, No. 15
Copyright © 1995, American Society for Microbiology

Comparison of a fungal (family I) and bacterial (family II) cellulose- binding domain

P Tomme, DP Driver, EA Amandoron, RC Miller Jr, R Antony, J Warren and DG Kilburn
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.

A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of cellobiohydrolase I (CbhI) from the fungus Trichoderma reesei. Expression of the hybrid gene in Escherichia coli yielded up to 50 mg of the hybrid protein, CexCBDCbhI, per liter of culture supernatant. The hybrid was purified to homogeneity by affinity chromatography on cellulose. The relative association constants (Kr) for the binding of Cex, CexCBDCbhI, the catalytic domain of Cex (p33), and CbhI to bacterial microcrystalline cellulose (BMCC) were 14.9, 7.8, 0.8, and 10.6 liters g-1, respectively. Cex and CexCBDCbhI had similar substrate specificities and similar activities on crystalline and amorphous cellulose. Both released predominantly cellobiose and cellotriose from amorphous cellulose. CexCBDCbhI was two to three times less active than Cex on BMCC, but significantly more active than Cex on soluble cellulose and on xylan. Unlike Cex, the hybrid protein neither bound to alpha-chitin nor released small particles from dewaxed cotton fibers.


This article has been cited by other articles:

  • Nimlos, M. R., Matthews, J. F., Crowley, M. F., Walker, R. C., Chukkapalli, G., Brady, J. W., Adney, W. S., Cleary, J. M., Zhong, L., Himmel, M. E. (2007). Molecular modeling suggests induced fit of Family I carbohydrate-binding modules with a broken-chain cellulose surface. Protein Eng Des Sel 0: gzm010v1-9 [Abstract] [Full Text]  
  • Poon, D. K. Y., Withers, S. G., McIntosh, L. P. (2007). Direct Demonstration of the Flexibility of the Glycosylated Proline-Threonine Linker in the Cellulomonas fimi Xylanase Cex through NMR Spectroscopic Analysis. J. Biol. Chem. 282: 2091-2100 [Abstract] [Full Text]  
  • Yoshida, K., Imaizumi, N., Kaneko, S., Kawagoe, Y., Tagiri, A., Tanaka, H., Nishitani, K., Komae, K. (2006). Carbohydrate-Binding Module of a Rice Endo-{beta}-1,4-glycanase, OsCel9A, Expressed in Auxin-Induced Lateral Root Primordia, is Post-Translationally Truncated. Plant Cell Physiol 47: 1555-1571 [Abstract] [Full Text]  
  • Shoseyov, O., Shani, Z., Levy, I. (2006). Carbohydrate Binding Modules: Biochemical Properties and Novel Applications. Microbiol. Mol. Biol. Rev. 70: 283-295 [Abstract] [Full Text]  
  • Nobles, D. R., Romanovicz, D. K., Brown, R. M. Jr. (2001). Cellulose in Cyanobacteria. Origin of Vascular Plant Cellulose Synthase?. Plant Physiol. 127: 529-542 [Abstract] [Full Text]  
  • Carrard, G., Koivula, A., Söderlund, H., Béguin, P. (2000). Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl. Acad. Sci. USA 10.1073/pnas.160216697v1 [Abstract] [Full Text]  
  • Hashimoto, M., Ikegami, T., Seino, S., Ohuchi, N., Fukada, H., Sugiyama, J., Shirakawa, M., Watanabe, T. (2000). Expression and Characterization of the Chitin-Binding Domain of Chitinase A1 from Bacillus circulans WL-12. J. Bacteriol. 182: 3045-3054 [Abstract] [Full Text]  
  • Jervis, E. J., Haynes, C. A., Kilburn, D. G. (1997). Surface Diffusion of Cellulases and Their Isolated Binding Domains on Cellulose. J. Biol. Chem. 272: 24016-24023 [Abstract] [Full Text]  
  • Linder, M., Salovuori, I., Ruohonen, L., Teeri, T. T. (1996). Characterization of a Double Cellulose-binding Domain. SYNERGISTIC HIGH AFFINITY BINDING TO CRYSTALLINE CELLULOSE. J. Biol. Chem. 271: 21268-21272 [Abstract] [Full Text]  
  • Carrard, G., Koivula, A., Soderlund, H., Beguin, P. (2000). Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl. Acad. Sci. USA 97: 10342-10347 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»