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J. Bacteriol., Aug 1995, 4544-4548, Vol 177, No. 15
Copyright © 1995, American Society for Microbiology

Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2)

DJ Bedford, E Schweizer, DA Hopwood and C Khosla
Department of Chemical Engineering, Stanford University, California 94305-5025, USA.

The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6- methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.


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