This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maupin-Furlow, J. A.
Right arrow Articles by Shanmugam, K. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maupin-Furlow, J. A.
Right arrow Articles by Shanmugam, K. T.

 Previous Article  |  Next Article 

J. Bacteriol., Sep 1995, 4851-4856, Vol 177, No. 17
Copyright © 1995, American Society for Microbiology

Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli

JA Maupin-Furlow, JK Rosentel, JH Lee, U Deppenmeier, RP Gunsalus and KT Shanmugam
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611, USA.

DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a potential membrane location, while the 352-amino-acid ModC protein (modC product) contains a nucleotide-binding motif. On the basis of sequence similarities with proteins from other transport systems and molybdate transport proteins from other organisms, these three proteins are proposed to constitute the molybdate transport system. The fourth open reading frame (modD) encodes a 231-amino-acid protein of unknown function. Plasmids containing different mod genes were used to map several molybdate- suppressible chlorate-resistant mutants; interestingly, none of the 40 mutants tested had a mutation in the modD gene. About 35% of these chlorate-resistant mutants were not complemented by mod operon DNA. These mutants, designated mol, contained mutations at unknown chromosomal location(s) and produced formate hydrogenlyase activity only when cultured in molybdate-supplemented glucose-minimal medium, not in L broth. This group of mol mutants constitutes a new class of molybdate utilization mutants distinct from other known mutants in molybdate metabolism. These results show that molybdate, after transport into cells by the ModABC proteins, is metabolized (activated?) by the products of the mol gene(s).


This article has been cited by other articles:

  • Koskiniemi, S., Andersson, D. I. (2009). Translesion DNA polymerases are required for spontaneous deletion formation in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 106: 10248-10253 [Abstract] [Full Text]  
  • Tejada-Jimenez, M., Llamas, A., Sanz-Luque, E., Galvan, A., Fernandez, E. (2007). A high-affinity molybdate transporter in eukaryotes. Proc. Natl. Acad. Sci. USA 104: 20126-20130 [Abstract] [Full Text]  
  • Delgado, M. J., Tresierra-Ayala, A., Talbi, C., Bedmar, E. J. (2006). Functional characterization of the Bradyrhizobium japonicum modA and modB genes involved in molybdenum transport. Microbiology 152: 199-207 [Abstract] [Full Text]  
  • KAISER, B. N., GRIDLEY, K. L., NGAIRE BRADY, J., PHILLIPS, T., TYERMAN, S. D. (2005). The Role of Molybdenum in Agricultural Plant Production. ANN BOT (LOND) 96: 745-754 [Abstract] [Full Text]  
  • Self, W. T., Hasona, A., Shanmugam, K. T. (2001). N-terminal truncations in the FhlA protein result in formate- and MoeA-independent expression of the hyc (formate hydrogenlyase) operon of Escherichia coli. Microbiology 147: 3093-3104 [Abstract] [Full Text]  
  • Grunden, A. M., Self, W. T., Villain, M., Blalock, J. E., Shanmugam, K. T. (1999). An Analysis of the Binding of Repressor Protein ModE to modABCD (Molybdate Transport) Operator/Promoter DNA of Escherichia coli. J. Biol. Chem. 274: 24308-24315 [Abstract] [Full Text]  
  • Wanner, C., Soppa, J. (1999). Genetic Identification of Three ABC Transporters as Essential Elements for Nitrate Respiration in Haloferax volcanii. Genetics 152: 1417-1428 [Abstract] [Full Text]  
  • Allen, R. M., Roll, J. T., Rangaraj, P., Shah, V. K., Roberts, G. P., Ludden, P. W. (1999). Incorporation of Molybdenum into the Iron-Molybdenum Cofactor of Nitrogenase. J. Biol. Chem. 274: 15869-15874 [Abstract] [Full Text]  
  • Guyer, D. M., Kao, J.-S., Mobley, H. L. T. (1998). Genomic Analysis of a Pathogenicity Island in Uropathogenic Escherichia coli CFT073: Distribution of Homologous Sequences among Isolates from Patients with Pyelonephritis, Cystitis, and CatheterAssociated Bacteriuria and from Fecal Samples. Infect. Immun. 66: 4411-4417 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Hasona, A., Ray, R. M., Shanmugam, K. T. (1998). Physiological and Genetic Analyses Leading to Identification of a Biochemical Role for the moeA (Molybdate Metabolism) Gene Product in Escherichia coli. J. Bacteriol. 180: 1466-1472 [Abstract] [Full Text]  
  • Rubio, L. M., Flores, E., Herrero, A. (1998). The narA Locus of Synechococcus sp. Strain PCC 7942 Consists of a Cluster of Molybdopterin Biosynthesis Genes. J. Bacteriol. 180: 1200-1206 [Abstract] [Full Text]  
  • Rech, S., Wolin, C., Gunsalus, R. P. (1996). Properties of the Periplasmic ModA Molybdate-binding Protein of Escherichia coli. J. Biol. Chem. 271: 2557-2562 [Abstract] [Full Text]  
  • Makdessi, K., Andreesen, J. R., Pich, A. (2001). Tungstate Uptake by a Highly Specific ABC Transporter in Eubacterium acidaminophilum. J. Biol. Chem. 276: 24557-24564 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»