This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, Y.
Right arrow Articles by Gabriel, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.
Right arrow Articles by Gabriel, D. W.

 Previous Article  |  Next Article 

J. Bacteriol., Sep 1995, 4963-4968, Vol 177, No. 17
Copyright © 1995, American Society for Microbiology

Intragenic recombination of a single plant pathogen gene provides a mechanism for the evolution of new host specificities

Y Yang and DW Gabriel
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville 32611, USA.

Gene pthA is required for virulence of Xanthomonas citri on citrus plants and has pleiotropic pathogenicity and avirulence functions when transferred to many different xanthomonads. DNA sequencing revealed that pthA belongs to a family of Xanthomonas avirulence/pathogenicity genes characterized by nearly identical 102-bp tandem repeats in the central region. By inserting an nptI-sac cartridge into the tandemly repeated region of pthA as a selective marker, intragenic recombination among homologous repeats was observed in both Xanthomonas spp. and Escherichia coli. Intragenic recombination within pthA created new genes with novel host specificities and altered pathogenicity and/or avirulence phenotypes. Many pthA recombinants gained or lost avirulence function in pathogenicity assays on bean, citrus, and cotton cultivars. Although the ability to induce cell division (hyperplastic cankers) on citrus could be lost, this ability was not acquired on cotton or bean plants. Intragenic recombination therefore provides a genetic mechanism for the generation of multiple, different, and gratuitous avirulence genes from a single, required, host-specific pathogenicity gene.


This article has been cited by other articles:

  • White, F. F., Yang, B. (2009). Host and Pathogen Factors Controlling the Rice-Xanthomonas oryzae Interaction. Plant Physiol. 150: 1677-1686 [Full Text]  
  • Heuer, H., Yin, Y.-N., Xue, Q.-Y., Smalla, K., Guo, J.-H. (2007). Repeat Domain Diversity of avrBs3-Like Genes in Ralstonia solanacearum Strains and Association with Host Preferences in the Field. Appl. Environ. Microbiol. 73: 4379-4384 [Abstract] [Full Text]  
  • Shiotani, H., Fujikawa, T., Ishihara, H., Tsuyumu, S., Ozaki, K. (2007). A pthA Homolog from Xanthomonas axonopodis pv. citri Responsible for Host-Specific Suppression of Virulence. J. Bacteriol. 189: 3271-3279 [Abstract] [Full Text]  
  • El Yacoubi, B., Brunings, A. M., Yuan, Q., Shankar, S., Gabriel, D. W. (2007). In Planta Horizontal Transfer of a Major Pathogenicity Effector Gene. Appl. Environ. Microbiol. 73: 1612-1621 [Abstract] [Full Text]  
  • Porter, B. W., Chittoor, J. M., Yano, M., Sasaki, T., White, F. F. (2003). Development and Mapping of Markers Linked to the Rice Bacterial Blight Resistance Gene Xa7. Crop Sci. 43: 1484-1492 [Abstract] [Full Text]  
  • Vera Cruz, C. M., Bai, J., Oña, I., Leung, H., Nelson, R. J., Mew, T.-W., Leach, J. E. (2000). Predicting durability of a disease resistance gene based on an assessment of the fitness loss and epidemiological consequences of avirulence gene mutation. Proc. Natl. Acad. Sci. USA 10.1073/pnas.250271997v1 [Abstract] [Full Text]  
  • Yang, B., Zhu, W., Johnson, L. B., White, F. F. (2000). The virulence factor AvrXa7 of Xanthomonas oryzae pv. oryzae is a type III secretion pathway-dependent nuclear-localized double-stranded DNA-binding protein. Proc. Natl. Acad. Sci. USA 10.1073/pnas.170286897v1 [Abstract] [Full Text]  
  • Zhu, W., Yang, B., Wills, N., Johnson, L. B., White, F. F. (1999). The C Terminus of AvrXa10 Can Be Replaced by the Transcriptional Activation Domain of VP16 from the Herpes Simplex Virus. Plant Cell 11: 1665-1674 [Abstract] [Full Text]  
  • Yang, Y, Shah, J, Klessig, D F (1997). Signal perception and transduction in plant defense responses.. Genes Dev. 11: 1621-1639  
  • Yang, B., Zhu, W., Johnson, L. B., White, F. F. (2000). The virulence factor AvrXa7 of Xanthomonas oryzae pv. oryzae is a type III secretion pathway-dependent nuclear-localized double-stranded DNA-binding protein. Proc. Natl. Acad. Sci. USA 97: 9807-9812 [Abstract] [Full Text]  
  • Vera Cruz, C. M., Bai, J., Ona, I., Leung, H., Nelson, R. J., Mew, T.-W., Leach, J. E. (2000). Predicting durability of a disease resistance gene based on an assessment of the fitness loss and epidemiological consequences of avirulence gene mutation. Proc. Natl. Acad. Sci. USA 97: 13500-13505 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»