This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gunn, J. S.
Right arrow Articles by Miller, S. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gunn, J. S.
Right arrow Articles by Miller, S. I.

 Previous Article  |  Next Article 

J. Bacteriol., Sep 1995, 5040-5047, Vol 177, No. 17
Copyright © 1995, American Society for Microbiology

Characterization of the Salmonella typhimurium pagC/pagD chromosomal region

JS Gunn, CM Alpuche-Aranda, WP Loomis, WJ Belden and SI Miller
Infectious Disease Unit, Massachusetts General Hospital, Boston, USA.

The PhoP/PhoQ two-component system regulates Salmonella typhimurium genes that are essential to bacterial virulence and survival within macrophages. The best characterized of these PhoP-activated genes (pag) is pagC, which encodes a 188-amino-acid envelope protein (W. S. Pulkkinen and S. I. Miller, J. Bacteriol. 173:86-93, 1991). We here report the identification of four genes (pagD, envE, msgA, and envF) located 5' to pagC. Each gene is transcribed from its own promoter, two of which (msgA and pagD) were defined by primer extension analysis. Three of these genes (pagD, envE, and envF) are predicted to encode envelope proteins. The pagD gene is transcribed in a direction opposite from that of and adjacent to pagC and is positively regulated by PhoP/PhoQ. Transposon insertions within pagD and msgA attenuate bacterial virulence and survival within macrophages; however, deletion of pagD has no effect on virulence. The product of the envF gene is predicted to be a lipoprotein on the basis of the presence of a consensus lipid attachment site. The low G + C content of these genes and the homology of msgA to Shigella plasmid DNA suggest that this region may have been acquired by horizontal transmission.


This article has been cited by other articles:

  • Zhao, G., Weatherspoon, N., Kong, W., Curtiss, R. III, Shi, Y. (2008). A dual-signal regulatory circuit activates transcription of a set of divergent operons in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 105: 20924-20929 [Abstract] [Full Text]  
  • Gerlach, R. G., Holzer, S. U., Jackel, D., Hensel, M. (2007). Rapid Engineering of Bacterial Reporter Gene Fusions by Using Red Recombination. Appl. Environ. Microbiol. 73: 4234-4242 [Abstract] [Full Text]  
  • Chiu, C.-H., Tang, P., Chu, C., Hu, S., Bao, Q., Yu, J., Chou, Y.-Y., Wang, H.-S., Lee, Y.-S. (2005). The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen. Nucleic Acids Res 33: 1690-1698 [Abstract] [Full Text]  
  • Chiu, C.-H., Su, L.-H., Chu, C. (2004). Salmonella enterica Serotype Choleraesuis: Epidemiology, Pathogenesis, Clinical Disease, and Treatment. Clin. Microbiol. Rev. 17: 311-322 [Abstract] [Full Text]  
  • Casjens, S. R., Gilcrease, E. B., Huang, W. M., Bunny, K. L., Pedulla, M. L., Ford, M. E., Houtz, J. M., Hatfull, G. F., Hendrix, R. W. (2004). The pKO2 Linear Plasmid Prophage of Klebsiella oxytoca. J. Bacteriol. 186: 1818-1832 [Abstract] [Full Text]  
  • Lejona, S., Aguirre, A., Cabeza, M. L., Vescovi, E. G., Soncini, F. C. (2003). Molecular Characterization of the Mg2+-Responsive PhoP-PhoQ Regulon in Salmonella enterica. J. Bacteriol. 185: 6287-6294 [Abstract] [Full Text]  
  • Norte, V. A., Stapleton, M. R., Green, J. (2003). PhoP-Responsive Expression of the Salmonella enterica Serovar Typhimurium slyA Gene. J. Bacteriol. 185: 3508-3514 [Abstract] [Full Text]  
  • Ho, T. D., Figueroa-Bossi, N., Wang, M., Uzzau, S., Bossi, L., Slauch, J. M. (2002). Identification of GtgE, a Novel Virulence Factor Encoded on the Gifsy-2 Bacteriophage of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 184: 5234-5239 [Abstract] [Full Text]  
  • Prouty, A. M., Van Velkinburgh, J. C., Gunn, J. S. (2002). Salmonella enterica Serovar Typhimurium Resistance to Bile: Identification and Characterization of the tolQRA Cluster. J. Bacteriol. 184: 1270-1276 [Abstract] [Full Text]  
  • Kim, B. H., Bang, I. S., Lee, S. Y., Hong, S. K., Bang, S. H., Lee, I. S., Park, Y. K. (2001). Expression of cspH, Encoding the Cold Shock Protein in Salmonella enterica Serovar Typhimurium UK-1. J. Bacteriol. 183: 5580-5588 [Abstract] [Full Text]  
  • Prouty, A. M., Gunn, J. S. (2000). Salmonella enterica Serovar Typhimurium Invasion Is Repressed in the Presence of Bile. Infect. Immun. 68: 6763-6769 [Abstract] [Full Text]  
  • Guina, T., Yi, E. C., Wang, H., Hackett, M., Miller, S. I. (2000). A PhoP-Regulated Outer Membrane Protease of Salmonella enterica Serovar Typhimurium Promotes Resistance to Alpha-Helical Antimicrobial Peptides. J. Bacteriol. 182: 4077-4086 [Abstract] [Full Text]  
  • Wei, Y., Miller, C. G. (1999). Characterization of a Group of Anaerobically Induced, fnr-Dependent Genes of Salmonella typhimurium. J. Bacteriol. 181: 6092-6097 [Abstract] [Full Text]  
  • Darwin, K. H., Miller, V. L. (1999). Molecular Basis of the Interaction of Salmonella with the Intestinal Mucosa. Clin. Microbiol. Rev. 12: 405-428 [Abstract] [Full Text]  
  • Vescovi, E. G., Ayala, Y. M., Di Cera, E., Groisman, E. A. (1997). Characterization of the Bacterial Sensor Protein PhoQ. EVIDENCE FOR DISTINCT BINDING SITES FOR Mg2+ AND Ca2+. J. Biol. Chem. 272: 1440-1443 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»