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J. Bacteriol., Jan 1995, 459-461, Vol 177, No. 2
Copyright © 1995, American Society for Microbiology

D-serine deaminase is a stringent selective marker in genetic crosses

WK Maas, R Maas and E McFall
Department of Microbiology, New York University School of Medicine, New York 10016.

The presence of the locus for D-serine deaminase (dsd) renders bacteria resistant to growth inhibition by D-serine and enables them to grow with D-serine as the sole nitrogen source. The two properties permit stringent selection in genetic crosses and make the D-serine deaminase gene an excellent marker, especially in the construction of strains for which the use of antibiotic resistance genes as selective markers is not allowed.


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