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J. Bacteriol., Oct 1995, 6018-6020, Vol 177, No. 20
Copyright © 1995, American Society for Microbiology

Hydrogenase does not confer significant benefits to Azotobacter vinelandii growing diazotrophically under conditions of glucose limitation

K Linkerhagner and J Oelze
Institut fur Biologie II (Mikrobiologie), Universitat Freiburg, Germany.

The presumed beneficial effect of hydrogenase on growth of diazotrophic bacteria was reinvestigated with carbon-limited chemostat cultures of the hydrogenase-deficient mutant hoxKG of Azotobacter vinelandii and its parent. The results revealed that hydrogen recycling was too low to benefit the cellular energy metabolism or activities of nitrogenase and respiration.

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