This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rouhiainen, L. Articles by Haselkorn, R. Search for Related Content PubMed PubMed Citation Articles by Rouhiainen, L. Articles by Haselkorn, R.

 Previous Article

J. Bacteriol., 10 1995, 6021-6026, Vol 177, No. 20
Copyright © 1995, American Society for Microbiology

Characterization of toxin-producing cyanobacteria by using an oligonucleotide probe containing a tandemly repeated heptamer

L Rouhiainen, K Sivonen, WJ Buikema and R Haselkorn
Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.

Cyanobacteria produce toxins that kill animals. The two main classes of cyanobacterial toxins are cyclic peptides that cause liver damage and alkaloids that block nerve transmission. Many toxin-producing strains from Finnish lakes were brought into axenic culture, and their toxins were characterized. Restriction fragment length polymorphism analysis, probing with a short tandemly repeated DNA sequence found at many locations in the chromosome of Anabaena sp. strain PCC 7120, distinguishes hepatotoxic Anabaena isolates from neurotoxin-producing strains and from Nostoc spp.

This article has been cited by other articles:

• Lyra, C., Laamanen, M., Lehtimaki, J. M., Surakka, A., Sivonen, K. (2005). Benthic cyanobacteria of the genus Nodularia are non-toxic, without gas vacuoles, able to glide and genetically more diverse than planktonic Nodularia. Int. J. Syst. Evol. Microbiol. 55: 555-568 [Abstract] [Full Text]  
• Rouhiainen, L., Vakkilainen, T., Siemer, B. L., Buikema, W., Haselkorn, R., Sivonen, K. (2004). Genes Coding for Hepatotoxic Heptapeptides (Microcystins) in the Cyanobacterium Anabaena Strain 90. Appl. Environ. Microbiol. 70: 686-692 [Abstract] [Full Text]  
• Vaitomaa, J., Rantala, A., Halinen, K., Rouhiainen, L., Tallberg, P., Mokelke, L., Sivonen, K. (2003). Quantitative Real-Time PCR for Determination of Microcystin Synthetase E Copy Numbers for Microcystis and Anabaena in Lakes. Appl. Environ. Microbiol. 69: 7289-7297 [Abstract] [Full Text]  
• Mikalsen, B., Boison, G., Skulberg, O. M., Fastner, J., Davies, W., Gabrielsen, T. M., Rudi, K., Jakobsen, K. S. (2003). Natural Variation in the Microcystin Synthetase Operon mcyABC and Impact on Microcystin Production in Microcystis Strains. J. Bacteriol. 185: 2774-2785 [Abstract] [Full Text]  
• Tillett, D., Parker, D. L., Neilan, B. A. (2001). Detection of Toxigenicity by a Probe for the Microcystin Synthetase A Gene (mcyA) of the Cyanobacterial Genus Microcystis: Comparison of Toxicities with 16S rRNA and Phycocyanin Operon (Phycocyanin Intergenic Spacer) Phylogenies. Appl. Environ. Microbiol. 67: 2810-2818 [Abstract] [Full Text]  
• Wilson, K. M., Schembri, M. A., Baker, P. D., Saint, C. P. (2000). Molecular Characterization of the Toxic Cyanobacterium Cylindrospermopsis raciborskii and Design of a Species-Specific PCR. Appl. Environ. Microbiol. 66: 332-338 [Abstract] [Full Text]  
• Rasmussen, U., Svenning, M. M. (1998). Fingerprinting of Cyanobacteria Based on PCR with Primers Derived from Short and Long Tandemly Repeated Repetitive Sequences. Appl. Environ. Microbiol. 64: 265-272 [Abstract] [Full Text]  
• Annila, A., Lehtimaki, J., Mattila, K., Eriksson, J. E., Sivonen, K., Rantala, T. T., Drakenberg, T. (1996). Solution Structure of Nodularin. AN INHIBITOR OF SERINE/THREONINE-SPECIFIC PROTEIN PHOSPHATASES. J. Biol. Chem. 271: 16695-16702 [Abstract] [Full Text]