This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wagner, E.
Right arrow Articles by Bruckner, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wagner, E.
Right arrow Articles by Bruckner, R.

 Previous Article  |  Next Article 

J. Bacteriol., 11 1995, 6144-6152, Vol 177, No. 21
Copyright © 1995, American Society for Microbiology

Glucose kinase-dependent catabolite repression in Staphylococcus xylosus

E Wagner, S Marcandier, O Egeter, J Deutscher, F Gotz and R Bruckner
Mikrobielle Genetik, Universitat Tubingen, Germany.

By transposon Tn917 mutagenesis, 16 mutants of Staphylococcus xylosus were isolated that showed higher levels of beta-galactosidase activity in the presence of glucose than the wild-type strain. The transposons were found to reside in three adjacent locations in the genome of S. xylosus. The nucleotide sequence of the chromosomal fragment affected by the Tn917 insertions yielded an open reading frame encoding a protein with a size of 328 amino acids with a high level of similarity to glucose kinase from Streptomyces coelicolor. Weaker similarity was also found to bacterial fructokinases and xylose repressors of gram- positive bacteria. The gene was designated glkA. Immediately downstream of glkA, two open reading frames were present whose deduced gene products showed no obvious similarity to known proteins. Measurements of catabolic enzyme activities in the mutant strains grown in the presence or absence of sugars established the pleiotropic nature of the mutations. Besides beta-galactosidase activity, which had been used to detect the mutants, six other tested enzymes were partially relieved from repression by glucose. Reduction of fructose-mediated catabolite repression was observed for some of the enzyme activities. Glucose transport and ATP-dependent phosphorylation of HPr, the phosphocarrier of the phosphoenolpyruvate:carbohydrate phosphotransferase system involved in catabolite repression in gram-positive bacteria, were not affected. The cloned glkA gene fully restored catabolite repression in the mutant strains in trans. Loss of GlkA function is thus responsible for the partial relief from catabolite repression. Glucose kinase activity in the mutants reached about 75% of the wild-type level, indicating the presence of another enzyme in S. xylosus.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:

  • Lu, G.-T., Yang, Z.-J., Peng, F.-Y., Tan, Y.-N., Tang, Y.-Q., Feng, J.-X., Tang, D.-J., He, Y.-Q., Tang, J.-L. (2007). The role of glucose kinase in carbohydrate utilization and extracellular polysaccharide production in Xanthomonas campestris pathovar campestris. Microbiology 153: 4284-4294 [Abstract] [Full Text]  
  • Saulnier, D. M. A., Molenaar, D., de Vos, W. M., Gibson, G. R., Kolida, S. (2007). Identification of Prebiotic Fructooligosaccharide Metabolism in Lactobacillus plantarum WCFS1 through Microarrays. Appl. Environ. Microbiol. 73: 1753-1765 [Abstract] [Full Text]  
  • Ryan, S. M., Fitzgerald, G. F., van Sinderen, D. (2005). Transcriptional Regulation and Characterization of a Novel {beta}-Fructofuranosidase-Encoding Gene from Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 71: 3475-3482 [Abstract] [Full Text]  
  • Brigham, C. J., Malamy, M. H. (2005). Characterization of the RokA and HexA Broad-Substrate-Specificity Hexokinases from Bacteroides fragilis and Their Role in Hexose and N-Acetylglucosamine Utilization. J. Bacteriol. 187: 890-901 [Abstract] [Full Text]  
  • Caescu, C. I., Vidal, O., Krzewinski, F., Artenie, V., Bouquelet, S. (2004). Bifidobacterium longum Requires a Fructokinase (Frk; ATP:D-Fructose 6-Phosphotransferase, EC 2.7.1.4) for Fructose Catabolism. J. Bacteriol. 186: 6515-6525 [Abstract] [Full Text]  
  • Dorr, C., Zaparty, M., Tjaden, B., Brinkmann, H., Siebers, B. (2003). The Hexokinase of the Hyperthermophile Thermoproteus tenax: ATP-DEPENDENT HEXOKINASES AND ADP-DEPENDENT GLUCOKINASES, TWO ALTERNATIVES FOR GLUCOSE PHOSPHORYLATION IN ARCHAEA. J. Biol. Chem. 278: 18744-18753 [Abstract] [Full Text]  
  • Hansen, T., Reichstein, B., Schmid, R., Schonheit, P. (2002). The First Archaeal ATP-Dependent Glucokinase, from the Hyperthermophilic Crenarchaeon Aeropyrum pernix, Represents a Monomeric, Extremely Thermophilic ROK Glucokinase with Broad Hexose Specificity. J. Bacteriol. 184: 5955-5965 [Abstract] [Full Text]  
  • Fields, M. W., Russell, J. B. (2001). The glucomannokinase of Prevotella bryantii B14 and its potential role in regulating {beta}-glucanase expression. Microbiology 147: 1035-1043 [Abstract] [Full Text]  
  • Jankovic, I., Egeter, O., Brückner, R. (2001). Analysis of Catabolite Control Protein A-Dependent Repression in Staphylococcus xylosus by a Genomic Reporter Gene System. J. Bacteriol. 183: 580-586 [Abstract] [Full Text]  
  • Weber, B. A., Klein, J. R., Henrich, B. (2000). Expression of the phospho-{beta}-glycosidase ArbZ from Lactobacillus delbrueckii subsp. lactis in Lactobacillus helveticus: substrate induction and catabolite repression. Microbiology 146: 1941-1948 [Abstract] [Full Text]  
  • Huynh, P. L., Jankovic, I., Schnell, N. F., Brückner, R. (2000). Characterization of an HPr Kinase Mutant of Staphylococcus xylosus. J. Bacteriol. 182: 1895-1902 [Abstract] [Full Text]  
  • Fiegler, H., Bassias, J., Jankovic, I., Brückner, R. (1999). Identification of a Gene in Staphylococcus xylosus Encoding a Novel Glucose Uptake Protein. J. Bacteriol. 181: 4929-4936 [Abstract] [Full Text]  
  • Zalieckas, J. M., Wray, L. V. Jr., Fisher, S. H. (1999). trans-Acting Factors Affecting Carbon Catabolite Repression of the hut Operon in Bacillus subtilis. J. Bacteriol. 181: 2883-2888 [Abstract] [Full Text]  
  • Behari, J., Youngman, P. (1998). A Homolog of CcpA Mediates Catabolite Control in Listeria monocytogenes but Not Carbon Source Regulation of Virulence Genes. J. Bacteriol. 180: 6316-6324 [Abstract] [Full Text]  
  • Behari, J., Youngman, P. (1998). Regulation of hly Expression in Listeria monocytogenes by Carbon Sources and pH Occurs through Separate Mechanisms Mediated by PrfA. Infect. Immun. 66: 3635-3642 [Abstract] [Full Text]  
  • Skarlatos, P., Dahl, M. K. (1998). . J. Bacteriol. 180: 3222-3226 [Abstract]  
  • Bassias, J., Brückner, R. (1998). Regulation of Lactose Utilization Genes in Staphylococcus xylosus. J. Bacteriol. 180: 2273-2279 [Abstract] [Full Text]  
  • Schönert, S., Buder, T., Dahl, M. K. (1998). Identification and Enzymatic Characterization of the Maltose-Inducible alpha -Glucosidase MalL (Sucrase-Isomaltase-Maltase) of Bacillus subtilis. J. Bacteriol. 180: 2574-2578 [Abstract] [Full Text]  
  • Ni, X., Westpheling, J. (1997). Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction. Proc. Natl. Acad. Sci. USA 94: 13116-13121 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»