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J. Bacteriol., Nov 1995, 6657-6665, Vol 177, No. 22
Copyright © 1995, American Society for Microbiology

Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC

MD Sutton and JM Kaguni
Department of Biochemistry, Michigan State University, East Lansing 48824-1319, USA.

Five novel alleles of the Escherichia coli dnaA gene that were temperature sensitive in maintenance of pSC101, a plasmid that is dependent on this gene for replication, were isolated. Nucleotide sequence analysis revealed that four of the five alleles arose from single base substitutions, whereas the fifth contained three base substitutions, two of which were silent. Whereas all five alleles were temperature sensitive in vivo for pSC101 maintenance, genetic and biochemical characterization indicated that only two were defective in replication from the chromosomal origin, oriC. As previously characterized mutations are defective in replication for both pSC101 and oriC, the dnaA mutations specifically defective in pSC101 maintenance represent a novel class. We speculate that one or more of these pSC101-specific mutants are defective in interaction with pSC101 RepA protein, which is also required for initiation of plasmid DNA replication.


This article has been cited by other articles:

  • Sutton, M. D. (2004). The Escherichia coli dnaN159 Mutant Displays Altered DNA Polymerase Usage and Chronic SOS Induction. J. Bacteriol. 186: 6738-6748 [Abstract] [Full Text]  
  • Miller, C., Ingmer, H., Thomsen, L. E., Skarstad, K., Cohen, S. N. (2003). DpiA Binding to the Replication Origin of Escherichia coli Plasmids and Chromosomes Destabilizes Plasmid Inheritance and Induces the Bacterial SOS Response. J. Bacteriol. 185: 6025-6031 [Abstract] [Full Text]  
  • Miller, C., Cohen, S. N. (1999). Separate Roles of Escherichia coli Replication Proteins in Synthesis and Partitioning of pSC101 Plasmid DNA. J. Bacteriol. 181: 7552-7557 [Abstract] [Full Text]  
  • Datta, H. J., Khatri, G. S., Bastia, D. (1999). Mechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101. Proc. Natl. Acad. Sci. USA 96: 73-78 [Abstract] [Full Text]  
  • Sutton, M. D., Carr, K. M., Vicente, M., Kaguni, J. M. (1998). Escherichia coli DnaA Protein. THE N-TERMINAL DOMAIN AND LOADING OF DnaB HELICASE AT THE E. COLI CHROMOSOMAL ORIGIN. J. Biol. Chem. 273: 34255-34262 [Abstract] [Full Text]  
  • Park, K., Mukhopadhyay, S., Chattoraj, D. K. (1998). Requirements for and Regulation of Origin Opening of Plasmid P1. J. Biol. Chem. 273: 24906-24911 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Sutton, M. D., Kaguni, J. M. (1997). Threonine 435 of Escherichia coli DnaA Protein Confers Sequence-specific DNA Binding Activity. J. Biol. Chem. 272: 23017-23024 [Abstract] [Full Text]  


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