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J. Bacteriol., 12 1995, 7265-7270, Vol 177, No. 24
Copyright © 1995, American Society for Microbiology

Characterization of Actinobacillus pleuropneumoniae riboflavin biosynthesis genes

TE Fuller and MH Mulks
Department of Microbiology, Michigan State University, East Lansing 48824, USA.

In this paper, we report the identification, cloning, and complete nucleotide sequence of four genes from Actinobacillus pleuropneumoniae that are involved in riboflavin biosynthesis. The cloned genes can specify production of large amounts of riboflavin in Escherichia coli, can complement several defined genetic mutations in riboflavin biosynthesis in E. coli, and are homologous to riboflavin biosynthetic genes from E. coli, Haemophilus influenzae, and Bacillus subtilis. The genes have been designated A. pleuropneumoniae ribGBAH because of their similarity in both sequence and arrangement to the B. subtilis ribGBAH operon.


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