This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Seoane, A. S.
Right arrow Articles by Levy, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seoane, A. S.
Right arrow Articles by Levy, S. B.

 Previous Article  |  Next Article 

J. Bacteriol., Feb 1995, 530-535, Vol 177, No. 3
Copyright © 1995, American Society for Microbiology

Identification of new genes regulated by the marRAB operon in Escherichia coli

AS Seoane and SB Levy
Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts 02111.

Random TnphoA and TnlacZ translational fusions were introduced into an Escherichia coli strain with a deletion of the multiple antibiotic resistance (mar) locus, complemented in trans by a temperature- sensitive plasmid bearing the mar locus with a constitutively expressed mar operon. Five gene fusions (two with lacZ and three with phoA) regulated by the mar operon were identified by increased or decreased marker enzyme activity following loss of the complementary plasmid at the restrictive temperature. Expression of LacZ from both lacZ fusions increased in the presence of the mar operon; expression from the three phoA fusions was represented by the mar operon. The lacZ fusions were mapped at 31.5 and 14 min on the Escherichia coli chromosome. One of the phoA fusions was located at 51.6 min while the two others mapped at 77 min. Cloning and sequencing of a portion of the fused genes showed all of them to be different. The phoA fusions at 77 min were located in a recently identified gene, slp, a lipoprotein of unknown function (D.M. Alexander and A. C. St. John, Mol. Microb. 11:1059-1071, 1994). The others showed no homology with any known genes of E. coli. The insertions caused small but reproducible changes in the antibiotic susceptibility profile. This approach has enabled the identification of new genes in E. coli which are regulated by the marRAB operon and involved in the Mar phenotype.


This article has been cited by other articles:

  • Lee, J. Y., Sung, B. H., Yu, B. J., Lee, J. H., Lee, S. H., Kim, M. S., Koob, M. D., Kim, S. C. (2008). Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli. Nucleic Acids Res 36: e102-e102 [Abstract] [Full Text]  
  • Udani, R. A., Levy, S. B. (2006). MarA-Like Regulator of Multidrug Resistance in Yersinia pestis.. Antimicrob. Agents Chemother. 50: 2971-2975 [Abstract] [Full Text]  
  • Prouty, A. M., Brodsky, I. E., Falkow, S., Gunn, J. S. (2004). Bile-salt-mediated induction of antimicrobial and bile resistance in Salmonella typhimurium. Microbiology 150: 775-783 [Abstract] [Full Text]  
  • Oliver, A., Weigel, L. M., Rasheed, J. K., McGowan, J. E. Jr., Raney, P., Tenover, F. C. (2002). Mechanisms of Decreased Susceptibility to Cefpodoxime in Escherichia coli. Antimicrob. Agents Chemother. 46: 3829-3836 [Abstract] [Full Text]  
  • Wassarman, K. M., Repoila, F., Rosenow, C., Storz, G., Gottesman, S. (2001). Identification of novel small RNAs using comparative genomics and microarrays. Genes Dev. 15: 1637-1651 [Abstract] [Full Text]  
  • Putman, M., van Veen, H. W., Konings, W. N. (2000). Molecular Properties of Bacterial Multidrug Transporters. Microbiol. Mol. Biol. Rev. 64: 672-693 [Abstract] [Full Text]  
  • Barbosa, T. M., Levy, S. B. (2000). Differential Expression of over 60 Chromosomal Genes in Escherichia coli by Constitutive Expression of MarA. J. Bacteriol. 182: 3467-3474 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Painbeni, E., Caroff, M., Rouviere-Yaniv, J. (1997). Alterations of the outer membrane composition in Escherichia coli lacking the histone-like protein HU. Proc. Natl. Acad. Sci. USA 94: 6712-6717 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»