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J. Bacteriol., Feb 1995, 851-853, Vol 177, No. 3
Copyright © 1995, American Society for Microbiology

Construction and plasmid-borne complementation of strains lacking the epsilon subunit of the Escherichia coli F1F0 ATP synthase

H Xiong and SB Vik
Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275.

Two strains of Escherichia coli that lack the epsilon subunit of the F1F0 ATP synthase have been constructed. They are shown to be viable but with very low growth yields (28%). These strains can be complemented by plasmids carrying wild-type uncC, but not when epsilon is overproduced. These results indicate that epsilon is not essential for growth on minimal glucose medium and that the level of its expression affects the assembly of the ATP synthase.

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