This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campbell, W. S.
Right arrow Articles by Laudenbach, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campbell, W. S.
Right arrow Articles by Laudenbach, D. E.

 Previous Article  |  Next Article 

J. Bacteriol., 02 1995, 964-972, Vol 177, No. 4
Copyright © 1995, American Society for Microbiology

Characterization of four superoxide dismutase genes from a filamentous cyanobacterium

WS Campbell and DE Laudenbach
Department of Plant Sciences, University of Western Ontario, London, Canada.

By using an oligonucleotide probe constructed from a conserved region of amino acids located in the carboxyl-terminal end of superoxide dismutase (SOD) proteins, four SOD genes were cloned from the cyanobacterium Plectonema boryanum UTEX 485. One of these genes, designated sodB, encoded an FeSOD enzyme, while the remaining three genes, designated sodA1, sodA2, and sodA3, encoded MnSOD enzymes. To investigate the expression of these four genes, total cellular RNA was isolated from P. boryanum UTEX 485 cells grown under various conditions and RNA gel blot analysis was carried out. Results indicated that sodB and sodA1 were constitutively expressed, although sodB expression was partially repressed in cells grown under conditions of iron stress. sodA2 transcripts, which were not detectable in control cells, accumulated to high levels in cells treated with methyl viologen or in cells grown under conditions of iron or nitrogen stress. However, under microaerobic conditions, iron and nitrogen stress failed to induce sodA2, indicating that multiple factors affect the regulation of sodA2. While discrete transcripts were not detected for sodA3, hybridization was observed under a number of conditions, including those which increased the accumulation of sodA2 transcripts. Additionally, there were high levels of the sodA3 transcript detected in a P. boryanum UTEX 485 mutant strain resistant to methyl viologen treatment.


This article has been cited by other articles:

  • Zhao, W., Guo, Q., Zhao, J. (2007). A Membrane-Associated Mn-Superoxide Dismutase Protects the Photosynthetic Apparatus and Nitrogenase from Oxidative Damage in the Cyanobacterium Anabaena sp. PCC 7120. Plant Cell Physiol 48: 563-572 [Abstract] [Full Text]  
  • Allen, M. D., Kropat, J., Tottey, S., Del Campo, J. A., Merchant, S. S. (2007). Manganese Deficiency in Chlamydomonas Results in Loss of Photosystem II and MnSOD Function, Sensitivity to Peroxides, and Secondary Phosphorus and Iron Deficiency. Plant Physiol. 143: 263-277 [Abstract] [Full Text]  
  • Regelsberger, G., Laaha, U., Dietmann, D., Ruker, F., Canini, A., Grilli-Caiola, M., Furtmuller, P. G., Jakopitsch, C., Peschek, G. A., Obinger, C. (2004). The Iron Superoxide Dismutase from the Filamentous Cyanobacterium Nostoc PCC 7120: LOCALIZATION, OVEREXPRESSION, AND BIOCHEMICAL CHARACTERIZATION. J. Biol. Chem. 279: 44384-44393 [Abstract] [Full Text]  
  • Regelsberger, G., Atzenhofer, W., Ruker, F., Peschek, G. A., Jakopitsch, C., Paumann, M., Furtmuller, P. G., Obinger, C. (2002). Biochemical Characterization of a Membrane-bound Manganese-containing Superoxide Dismutase from the Cyanobacterium Anabaena PCC 7120. J. Biol. Chem. 277: 43615-43622 [Abstract] [Full Text]  
  • Li, T., Huang, X., Zhou, R., Liu, Y., Li, B., Nomura, C., Zhao, J. (2002). Differential Expression and Localization of Mn and Fe Superoxide Dismutases in the Heterocystous Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 5096-5103 [Abstract] [Full Text]  
  • Shirkey, B., Kovarcik, D. P., Wright, D. J., Wilmoth, G., Prickett, T. F., Helm, R. F., Gregory, E. M., Potts, M. (2000). Active Fe-Containing Superoxide Dismutase and Abundant sodF mRNA in Nostoc commune (Cyanobacteria) after Years of Desiccation. J. Bacteriol. 182: 189-197 [Abstract] [Full Text]  
  • Tichy, M., Vermaas, W. (1999). In Vivo Role of Catalase-Peroxidase in Synechocystis sp. Strain PCC 6803. J. Bacteriol. 181: 1875-1882 [Abstract] [Full Text]  
  • Cortez, N., Carrillo, N., Pasternak, C., Balzer, A., Klug, G. (1998). Molecular Cloning and Expression Analysis of the Rhodobacter capsulatus sodB Gene, Encoding an Iron Superoxide Dismutase. J. Bacteriol. 180: 5413-5420 [Abstract] [Full Text]  
  • Celerin, M., Gilpin, A. A., Schisler, N. J., Ivanov, A. G., Miskiewicz, E., Krol, M., Laudenbach, D. E. (1998). ClpB in a Cyanobacterium: Predicted Structure, Phylogenetic Relationships, and Regulation by Light and Temperature. J. Bacteriol. 180: 5173-5182 [Abstract] [Full Text]  
  • Thomas, D. J., Avenson, T. J., Thomas, J. B., Herbert, S. K. (1998). A Cyanobacterium Lacking Iron Superoxide Dismutase Is Sensitized to Oxidative Stress Induced with Methyl Viologen but Is Not Sensitized to Oxidative Stress Induced with Norflurazon. Plant Physiol. 116: 1593-1602 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»