This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palmer, J. R.
Right arrow Articles by Daniels, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palmer, J. R.
Right arrow Articles by Daniels, C. J.

 Previous Article  |  Next Article 

J. Bacteriol., 04 1995, 1844-1849, Vol 177, No. 7
Copyright © 1995, American Society for Microbiology

In vivo definition of an archaeal promoter

JR Palmer and CJ Daniels
Department of Microbiology, Ohio State University, Columbus 43210.

We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding to the TATA-like region were altered. Promoters with wild-type or higher levels of transcriptional activity conformed to the general archaeal box A consensus, 5'-T/CTTAT/AA-3'. The preference for a pyrimidine residue in the 5' position of this region and the exclusion of guanine and cytosine in the next four positions in the 3' direction are defining characteristics shared by all efficient archaeal promoters. We have also observed that replacement of a 10-nucleotide purine-rich sequence, located 5' of the H. volcanii tRNA(Lys) box A element, completely abolished transcription from this promoter. These data show that the H. volcanii tRNA(Lys) promoter is dependent on two separate, and essential, sequence elements. The possible functions of these sequences, in view of the recent descriptions of eucaryal-like transcription factors for Archaea, are discussed.


This article has been cited by other articles:

  • Zhang, J., Li, E., Olsen, G. J. (2009). Protein-coding gene promoters in Methanocaldococcus (Methanococcus) jannaschii. Nucleic Acids Res 37: 3588-3601 [Abstract] [Full Text]  
  • Micorescu, M., Grunberg, S., Franke, A., Cramer, P., Thomm, M., Bartlett, M. (2008). Archaeal Transcription: Function of an Alternative Transcription Factor B from Pyrococcus furiosus. J. Bacteriol. 190: 157-167 [Abstract] [Full Text]  
  • Johnsen, U., Schonheit, P. (2004). Novel Xylose Dehydrogenase in the Halophilic Archaeon Haloarcula marismortui. J. Bacteriol. 186: 6198-6207 [Abstract] [Full Text]  
  • Serganova, I., Ksenzenko, V., Serganov, A., Meshcheryakova, I., Pyatibratov, M., Vakhrusheva, O., Metlina, A., Fedorov, O. (2002). Sequencing of Flagellin Genes from Natrialba magadii Provides New Insight into Evolutionary Aspects of Archaeal Flagellins. J. Bacteriol. 184: 318-322 [Abstract] [Full Text]  
  • Empadinhas, N., Marugg, J. D., Borges, N., Santos, H., da Costa, M. S. (2001). Pathway for the Synthesis of Mannosylglycerate in the Hyperthermophilic Archaeon Pyrococcus horikoshii. BIOCHEMICAL AND GENETIC CHARACTERIZATION OF KEY ENZYMES. J. Biol. Chem. 276: 43580-43588 [Abstract] [Full Text]  
  • Wendoloski, D., Ferrer, C., Dyall-Smith, M. L. (2001). A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2. Microbiology 147: 959-964 [Abstract] [Full Text]  
  • Ishikawa, K., Ishida, H., Matsui, I., Kawarabayasi, Y., Kikuchi, H. (2001). Novel Bifunctional Hyperthermostable Carboxypeptidase/Aminoacylase from Pyrococcus horikoshii OT3. Appl. Environ. Microbiol. 67: 673-679 [Abstract] [Full Text]  
  • Price, L. B., Shand, R. F. (2000). Halocin S8: a 36-Amino-Acid Microhalocin from the Haloarchaeal Strain S8a. J. Bacteriol. 182: 4951-4958 [Abstract] [Full Text]  
  • Enoru-Eta, J., Gigot, D., Thia-Toong, T.-L., Glansdorff, N., Charlier, D. (2000). Purification and Characterization of Sa-Lrp, a DNA-Binding Protein from the Extreme Thermoacidophilic Archaeon Sulfolobus acidocaldarius Homologous to the Bacterial Global Transcriptional Regulator Lrp. J. Bacteriol. 182: 3661-3672 [Abstract] [Full Text]  
  • Jolley, K. A., Maddocks, D. G., Gyles, S. L., Mullan, Z., Tang, S.-L., Dyall-Smith, M. L., Hough, D. W., Danson, M. J. (2000). 2-Oxoacid dehydrogenase multienzyme complexes in the halophilic Archaea? Gene sequences and protein structural predictions. Microbiology 146: 1061-1069 [Abstract] [Full Text]  
  • Haseltine, C., Montalvo-Rodriguez, R., Bini, E., Carl, A., Blum, P. (1999). Coordinate Transcriptional Control in the Hyperthermophilic Archaeon Sulfolobus solfataricus. J. Bacteriol. 181: 3920-3927 [Abstract] [Full Text]  
  • Voorhorst, W. G. B., Gueguen, Y., Geerling, A. C. M., Schut, G., Dahlke, I., Thomm, M., van der Oost, J., de Vos, W. M. (1999). Transcriptional Regulation in the Hyperthermophilic Archaeon Pyrococcus furiosus: Coordinated Expression of Divergently Oriented Genes in Response to beta -Linked Glucose Polymers. J. Bacteriol. 181: 3777-3783 [Abstract] [Full Text]  
  • Baliga, N. S., DasSarma, S. (1999). Saturation Mutagenesis of the TATA Box and Upstream Activator Sequence in the Haloarchaeal bop Gene Promoter. J. Bacteriol. 181: 2513-2518 [Abstract] [Full Text]  
  • Schleper, C., DeLong, E. F., Preston, C. M., Feldman, R. A., Wu, K.-Y., Swanson, R. V. (1998). Genomic Analysis Reveals Chromosomal Variation in Natural Populations of the Uncultured Psychrophilic Archaeon Cenarchaeum symbiosum. J. Bacteriol. 180: 5003-5009 [Abstract] [Full Text]  
  • Nomura, N., Sako, Y., Uchida, A. (1998). Molecular Characterization and Postsplicing Fate of Three Introns within the Single rRNA Operon of the Hyperthermophilic Archaeon Aeropyrum pernix K1. J. Bacteriol. 180: 3635-3643 [Abstract] [Full Text]  
  • Ishikawa, K., Ishida, H., Koyama, Y., Kawarabayasi, Y., Kawahara, J.-i., Matsui, E., Matsui, I. (1998). Acylamino Acid-releasing Enzyme from the Thermophilic Archaeon Pyrococcus horikoshii. J. Biol. Chem. 273: 17726-17731 [Abstract] [Full Text]  
  • Burke, S. A., Lo, S. L., Krzycki, J. A. (1998). Clustered Genes Encoding the Methyltransferases of Methanogenesis from Monomethylamine. J. Bacteriol. 180: 3432-3440 [Abstract] [Full Text]  
  • Chien, Y.-t., Helmann, J. D., Zinder, S. H. (1998). Interactions between the Promoter Regions of Nitrogenase Structural Genes (nifHDK2) and DNA-Binding Proteins from N2- and Ammonium-Grown Cells of the Archaeon Methanosarcina barkeri 227. J. Bacteriol. 180: 2723-2728 [Abstract] [Full Text]  
  • BELL, S.D., JACKSON, S.P. (1998). Transcription in Archaea. Cold Spring Harb Symp Quant Biol 63: 41-52 [Abstract]  
  • Armbruster, D. W., Daniels, C. J. (1997). Splicing of Intron-containing tRNATrp by the Archaeon Haloferax volcanii Occurs Independent of Mature tRNA Structure. J. Biol. Chem. 272: 19758-19762 [Abstract] [Full Text]  
  • Kosa, P. F., Ghosh, G., DeDecker, B. S., Sigler, P. B. (1997). The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box. Proc. Natl. Acad. Sci. USA 94: 6042-6047 [Abstract] [Full Text]  
  • LeClerc, G. M., Grahame, D. A. (1996). Methylcobamide:Coenzyme M Methyltransferase Isozymes from Methanosarcina barkeri. PHYSICOCHEMICAL CHARACTERIZATION, CLONING, SEQUENCE ANALYSIS, AND HETEROLOGOUS GENE EXPRESSION. J. Biol. Chem. 271: 18725-18731 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»