This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de la Cruz, J.
Right arrow Articles by Llobell, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de la Cruz, J.
Right arrow Articles by Llobell, A.

 Previous Article  |  Next Article 

J. Bacteriol., Apr 1995, 1864-1871, Vol 177, No. 7
Copyright © 1995, American Society for Microbiology

Purification and characterization of an endo-beta-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism

J de la Cruz, JA Pintor-Toro, T Benitez and A Llobell
Instituto de Bioquimica Vegetal y Fotosintesis, Facultad de Biologia, Universidad de Sevilla, Spain.

The enzymes from Trichoderma species that degrade fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal plant pathogens. The mycoparasite Trichoderma harzianum produces at least two extracellular beta-1,6-glucanases, among other hydrolases, when it is grown on chitin as the sole carbon source. One of these extracellular enzymes was purified to homogeneity after adsorption to its substrate, pustulan, chromatofocusing, and, finally, gel filtration. The apparent molecular mass was 43,000, and the isoelectric point was 5.8. The first 15 amino acids from the N terminus of the purified protein have been sequenced. The enzyme was specific for beta-1,6 linkages and showed an endolytic mode of action on pustulan. Further characterization indicated that the enzyme by itself releases soluble sugars and produces hydrolytic halli on yeast cell walls. When combined with other T. harzianum cell wall-degrading enzymes such as beta-1,3-glucanases and chitinases, it hydrolyzes filamentous fungal cell walls. The enzyme acts cooperatively with the latter enzymes, inhibiting the growth of the fungi tested. Antibodies against the purified protein also indicated that the two identified beta-1,6-glucanases are not immunologically related and are probably encoded by two different genes.


This article has been cited by other articles:

  • Aimanianda, V., Clavaud, C., Simenel, C., Fontaine, T., Delepierre, M., Latge, J.-P. (2009). Cell Wall {beta}-(1,6)-Glucan of Saccharomyces cerevisiae: STRUCTURAL CHARACTERIZATION AND IN SITU SYNTHESIS. J. Biol. Chem. 284: 13401-13412 [Abstract] [Full Text]  
  • Fortwendel, J. R., Fuller, K. K., Stephens, T. J., Bacon, W. C., Askew, D. S., Rhodes, J. C. (2008). Aspergillus fumigatus RasA Regulates Asexual Development and Cell Wall Integrity. Eukaryot Cell 7: 1530-1539 [Abstract] [Full Text]  
  • Djonovic, S., Pozo, M. J., Kenerley, C. M. (2006). Tvbgn3, a {beta}-1,6-Glucanase from the Biocontrol Fungus Trichoderma virens, Is Involved in Mycoparasitism and Control of Pythium ultimum. Appl. Environ. Microbiol. 72: 7661-7670 [Abstract] [Full Text]  
  • Palma, A. S., Feizi, T., Zhang, Y., Stoll, M. S., Lawson, A. M., Diaz-Rodriguez, E., Campanero-Rhodes, M. A., Costa, J., Gordon, S., Brown, G. D., Chai, W. (2006). Ligands for the beta-Glucan Receptor, Dectin-1, Assigned Using "Designer" Microarrays of Oligosaccharide Probes (Neoglycolipids) Generated from Glucan Polysaccharides. J. Biol. Chem. 281: 5771-5779 [Abstract] [Full Text]  
  • Lutz, M. P., Feichtinger, G., Defago, G., Duffy, B. (2003). Mycotoxigenic Fusarium and Deoxynivalenol Production Repress Chitinase Gene Expression in the Biocontrol Agent Trichoderma atroviride P1. Appl. Environ. Microbiol. 69: 3077-3084 [Abstract] [Full Text]  
  • Moy, M., Li, H. M., Sullivan, R., White, J. F. Jr., Belanger, F. C. (2002). Endophytic Fungal beta -1,6-Glucanase Expression in the Infected Host Grass. Plant Physiol. 130: 1298-1308 [Abstract] [Full Text]  
  • Delgado-Jarana, J., Rincon, A. M., Benitez, T. (2002). Aspartyl protease from Trichoderma harzianum CECT 2413: cloning and characterization. Microbiology 148: 1305-1315 [Abstract] [Full Text]  
  • Ait-Lahsen, H., Soler, A., Rey, M., de la Cruz, J., Monte, E., Llobell, A. (2001). An Antifungal Exo-alpha -1,3-Glucanase (AGN13.1) from the Biocontrol Fungus Trichoderma harzianum. Appl. Environ. Microbiol. 67: 5833-5839 [Abstract] [Full Text]  
  • Pavia, J., Aguado, C., Mormeneo, S., Sentandreu, R. (2001). Secretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans. Microbiology 147: 1983-1991 [Abstract] [Full Text]  
  • Ram, A. F. J., Kapteyn, J. C., Montijn, R. C., Caro, L. H. P., Douwes, J. E., Baginsky, W., Mazur, P., van den Ende, H., Klis, F. M. (1998). Loss of the Plasma Membrane-Bound Protein Gas1p in Saccharomyces cerevisiae Results in the Release of beta 1,3-Glucan into the Medium and Induces a Compensation Mechanism To Ensure Cell Wall Integrity. J. Bacteriol. 180: 1418-1424 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»