J. Bacteriol., Apr 1995, 1915-1917, Vol 177, No. 7
S Futo, Y Seto, S Mitsuse, Y Mori, T Suzuki and K Kawai
The DNA sequence of the gene encoding the early and specific 46-kDa surface
antigen (P46) of Mycoplasma hyopneumoniae has been determined. The P46
gene, encoding a putative lipoprotein, contained three TGA codons and a
single CGG codon in a 1,257-bp open reading frame. Edman degradation of
peptide fragments showed that at least one TGA codon encodes tryptophan and
that the CGG codon, which has been reported to be nonsense or unassigned in
other mycoplasmas, is used for arginine in M. hyopneumoniae.
Copyright © 1995, American Society for Microbiology
Molecular cloning of a 46-kilodalton surface antigen (P46) gene from Mycoplasma hyopneumoniae: direct evidence of CGG codon usage for arginine
United Graduate School of Agricultural Sciences, Faculty of Agriculture, Gifu University, Japan.
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