This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Garcia, M. M. Articles by Blaser, M. J. Search for Related Content PubMed PubMed Citation Articles by Garcia, M. M. Articles by Blaser, M. J.

 Previous Article  |  Next Article 

J. Bacteriol., 04 1995, 1976-1980, Vol 177, No. 8
Copyright © 1995, American Society for Microbiology

Protein shift and antigenic variation in the S-layer of Campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapA homologs

MM Garcia, CL Lutze-Wallace, AS Denes, MD Eaglesome, E Holst and MJ Blaser
Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario.

Campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a C. fetus-negative heifer. Isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kDa S-layer protein. Immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kDa S-layer protein and other C. fetus S-layer proteins demonstrated antigenic shifts. Genomic digests of the isolates probed with a 75-mer oligonucleotide of the conserved sapA region also indicated that antigenic variation of the S-layer is accompanied by DNA rearrangement.

This article has been cited by other articles:

• Hansmeier, N., Albersmeier, A., Tauch, A., Damberg, T., Ros, R., Anselmetti, D., Puhler, A., Kalinowski, J. (2006). The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032.. Microbiology 152: 923-935 [Abstract] [Full Text]  
• Tu, Z.-C., Hui, J., Blaser, M. J. (2004). Conservation and Diversity of sap Homologues and Their Organization among Campylobacter fetus Isolates. Infect. Immun. 72: 1715-1724 [Abstract] [Full Text]  
• Patterson, M. M., O'Toole, P. W., Forester, N. T., Noonan, B., Trust, T. J., Xu, S., Taylor, N. S., Marini, R. P., Ihrig, M. M., Fox, J. G. (2003). Failure of Surface Ring Mutant Strains of Helicobacter mustelae To Persistently Infect the Ferret Stomach. Infect. Immun. 71: 2350-2355 [Abstract] [Full Text]  
• Grogono-Thomas, R., Blaser, M. J., Ahmadi, M., Newell, D. G. (2003). Role of S-Layer Protein Antigenic Diversity in the Immune Responses of Sheep Experimentally Challenged with Campylobacter fetus subsp. fetus. Infect. Immun. 71: 147-154 [Abstract] [Full Text]  
• Lysnyansky, I., Ron, Y., Sachse, K., Yogev, D. (2001). Intrachromosomal Recombination within the vsp Locus of Mycoplasma bovis Generates a Chimeric Variable Surface Lipoprotein Antigen. Infect. Immun. 69: 3703-3712 [Abstract] [Full Text]  
• Grogono-Thomas, R., Dworkin, J., Blaser, M. J., Newell, D. G. (2000). Roles of the Surface Layer Proteins of Campylobacter fetus subsp. fetus in Ovine Abortion. Infect. Immun. 68: 1687-1691 [Abstract] [Full Text]  
• Soual-Hoebeke, E., Sousa- D'Auria, C. d., Chami, M., Baucher, M.-F., Guyonvarch, A., Bayan, N., Salim, K., Leblon, G. (1999). S-layer protein production by Corynebacterium strains is dependent on the carbon source. Microbiology 145: 3399-3408 [Abstract] [Full Text]  
• Wassenaar, T. M., Geilhausen, B., Newell, D. G. (1998). Evidence of Genomic Instability in Campylobacter jejuni Isolated from Poultry. Appl. Environ. Microbiol. 64: 1816-1821 [Abstract] [Full Text]  
• Dworkin, J., Blaser, M. J. (1997). Nested DNA inversion as a paradigm of programmed gene rearrangement. Proc. Natl. Acad. Sci. USA 94: 985-990 [Abstract] [Full Text]