This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lemaire, M.
Right arrow Articles by Beguin, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemaire, M.
Right arrow Articles by Beguin, P.

 Previous Article  |  Next Article 

J. Bacteriol., May 1995, 2451-2459, Vol 177, No. 9
Copyright © 1995, American Society for Microbiology

OlpB, a new outer layer protein of Clostridium thermocellum, and binding of its S-layer-like domains to components of the cell envelope

M Lemaire, H Ohayon, P Gounon, T Fujino and P Beguin
Unite de Physiologie Cellulaire and URA 1300 CNRS, Departement des Biotechnologies, Paris, France.

Several proteins of Clostridium thermocellum possess a C-terminal triplicated sequence related to bacterial cell surface proteins. This sequence was named the SLH domain (for S-layer homology), and it was proposed that it might serve to anchor proteins to the cell surface (A. Lupas, H. Engelhardt, J. Peters, U. Santarius, S. Volker, and W. Baumeister, J. Bacteriol. 176:1224-1233, 1994). This hypothesis was investigated by using the SLH-containing protein ORF1p from C. thermocellum as a model. Subcellular fractionation, immunoblotting, and electron microscopy of immunocytochemically labeled cells indicated that ORF1p was located on the surface of C. thermocellum. To detect C. thermocellum components interacting with the SLH domains of ORF1p, a probe was constructed by grafting these domains on the C terminus of the MalE protein of Escherichia coli. The SLH domains conferred on the chimeric protein (MalE-ORF1p-C) the ability to bind noncovalently to the peptidoglycan of C. thermocellum. In addition, 125I-labeled MalE- ORF1p-C was shown to bind to SLH-bearing proteins transferred onto nitrocellulose, and to a 26- to 28-kDa component of the cell envelope. These results agree with the hypothesis that SLH domains contribute to the binding of exocellular proteins to the cell surface of bacteria. The gene carrying ORF1 and its product, ORF1p, are renamed olpB and OlpB (for outer layer protein B), respectively.


This article has been cited by other articles:

  • Rice, K. C., Bayles, K. W. (2008). Molecular Control of Bacterial Death and Lysis. Microbiol. Mol. Biol. Rev. 72: 85-109 [Abstract] [Full Text]  
  • Gold, N. D., Martin, V. J. J. (2007). Global View of the Clostridium thermocellum Cellulosome Revealed by Quantitative Proteomic Analysis. J. Bacteriol. 189: 6787-6795 [Abstract] [Full Text]  
  • Matsumi, R., Manabe, K., Fukui, T., Atomi, H., Imanaka, T. (2007). Disruption of a Sugar Transporter Gene Cluster in a Hyperthermophilic Archaeon Using a Host-Marker System Based on Antibiotic Resistance. J. Bacteriol. 189: 2683-2691 [Abstract] [Full Text]  
  • Adams, J. J., Pal, G., Jia, Z., Smith, S. P. (2006). Mechanism of bacterial cell-surface attachment revealed by the structure of cellulosomal type II cohesin-dockerin complex. Proc. Natl. Acad. Sci. USA 103: 305-310 [Abstract] [Full Text]  
  • Rincon, M. T., Cepeljnik, T., Martin, J. C., Lamed, R., Barak, Y., Bayer, E. A., Flint, H. J. (2005). Unconventional Mode of Attachment of the Ruminococcus flavefaciens Cellulosome to the Cell Surface. J. Bacteriol. 187: 7569-7578 [Abstract] [Full Text]  
  • Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  
  • Kosugi, A., Amano, Y., Murashima, K., Doi, R. H. (2004). Hydrophilic Domains of Scaffolding Protein CbpA Promote Glycosyl Hydrolase Activity and Localization of Cellulosomes to the Cell Surface of Clostridium cellulovorans. J. Bacteriol. 186: 6351-6359 [Abstract] [Full Text]  
  • Xu, Q., Barak, Y., Kenig, R., Shoham, Y., Bayer, E. A., Lamed, R. (2004). A Novel Acetivibrio cellulolyticus Anchoring Scaffoldin That Bears Divergent Cohesins. J. Bacteriol. 186: 5782-5789 [Abstract] [Full Text]  
  • Rincon, M. T., Martin, J. C., Aurilia, V., McCrae, S. I., Rucklidge, G. J., Reid, M. D., Bayer, E. A., Lamed, R., Flint, H. J. (2004). ScaC, an Adaptor Protein Carrying a Novel Cohesin That Expands the Dockerin-Binding Repertoire of the Ruminococcus flavefaciens 17 Cellulosome. J. Bacteriol. 186: 2576-2585 [Abstract] [Full Text]  
  • Xu, Q., Bayer, E. A., Goldman, M., Kenig, R., Shoham, Y., Lamed, R. (2004). Architecture of the Bacteroides cellulosolvens Cellulosome: Description of a Cell Surface-Anchoring Scaffoldin and a Family 48 Cellulase. J. Bacteriol. 186: 968-977 [Abstract] [Full Text]  
  • Doi, R. H., Kosugi, A., Murashima, K., Tamaru, Y., Han, S. O. (2003). Cellulosomes from Mesophilic Bacteria. J. Bacteriol. 185: 5907-5914 [Full Text]  
  • Dror, T. W., Rolider, A., Bayer, E. A., Lamed, R., Shoham, Y. (2003). Regulation of Expression of Scaffoldin-Related Genes in Clostridium thermocellum. J. Bacteriol. 185: 5109-5116 [Abstract] [Full Text]  
  • Xu, Q., Gao, W., Ding, S.-Y., Kenig, R., Shoham, Y., Bayer, E. A., Lamed, R. (2003). The Cellulosome System of Acetivibrio cellulolyticus Includes a Novel Type of Adaptor Protein and a Cell Surface Anchoring Protein. J. Bacteriol. 185: 4548-4557 [Abstract] [Full Text]  
  • Fuchs, K.-P., Zverlov, V. V., Velikodvorskaya, G. A., Lottspeich, F., Schwarz, W. H. (2003). Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-{beta}-1,3-glucanase bound to the outer cell surface. Microbiology 149: 1021-1031 [Abstract] [Full Text]  
  • Rincon, M. T., Ding, S.-Y., McCrae, S. I., Martin, J. C., Aurilia, V., Lamed, R., Shoham, Y., Bayer, E. A., Flint, H. J. (2003). Novel Organization and Divergent Dockerin Specificities in the Cellulosome System of Ruminococcus flavefaciens. J. Bacteriol. 185: 703-713 [Abstract] [Full Text]  
  • Koksharova, O. A., Wolk, C. P. (2002). Novel DNA-Binding Proteins in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 3931-3940 [Abstract] [Full Text]  
  • Kosugi, A., Murashima, K., Tamaru, Y., Doi, R. H. (2002). Cell-Surface-Anchoring Role of N-Terminal Surface Layer Homology Domains of Clostridium cellulovorans EngE. J. Bacteriol. 184: 884-888 [Abstract] [Full Text]  
  • Ding, S.-Y., Rincon, M. T., Lamed, R., Martin, J. C., McCrae, S. I., Aurilia, V., Shoham, Y., Bayer, E. A., Flint, H. J. (2001). Cellulosomal Scaffoldin-Like Proteins from Ruminococcus flavefaciens. J. Bacteriol. 183: 1945-1953 [Abstract] [Full Text]  
  • Ding, S.-Y., Bayer, E. A., Steiner, D., Shoham, Y., Lamed, R. (2000). A Scaffoldin of the Bacteroides cellulosolvens Cellulosome That Contains 11 Type II Cohesins. J. Bacteriol. 182: 4915-4925 [Abstract] [Full Text]  
  • Sára, M., Sleytr, U. B. (2000). S-Layer Proteins. J. Bacteriol. 182: 859-868 [Full Text]  
  • Brechtel, E., Bahl, H. (1999). In Thermoanaerobacterium thermosulfurigenes EM1 S-Layer Homology Domains Do Not Attach to Peptidoglycan. J. Bacteriol. 181: 5017-5023 [Abstract] [Full Text]  
  • Stoll, D., Stålbrand, H., Warren, R. A. J. (1999). Mannan-Degrading Enzymes from Cellulomonas fimi. Appl. Environ. Microbiol. 65: 2598-2605 [Abstract] [Full Text]  
  • Erra-Pujada, M., Debeire, P., Duchiron, F., O'Donohue, M. J. (1999). The Type II Pullulanase of Thermococcus hydrothermalis: Molecular Characterization of the Gene and Expression of the Catalytic Domain. J. Bacteriol. 181: 3284-3287 [Abstract] [Full Text]  
  • Chauvaux, S., Matuschek, M., Beguin, P. (1999). Distinct Affinity of Binding Sites for S-Layer Homologous Domains in Clostridium thermocellum and Bacillus anthracis Cell Envelopes. J. Bacteriol. 181: 2455-2458 [Abstract] [Full Text]  
  • Pagès, S., Bélaïch, A., Fierobe, H.-P., Tardif, C., Gaudin, C., Bélaïch, J.-P. (1999). Sequence Analysis of Scaffolding Protein CipC and ORFXp, a New Cohesin-Containing Protein in Clostridium cellulolyticum: Comparison of Various Cohesin Domains and Subcellular Localization of ORFXp. J. Bacteriol. 181: 1801-1810 [Abstract] [Full Text]  
  • Cann, I. K. O., Kocherginskaya, S., King, M. R., White, B. A., Mackie, R. I. (1999). Molecular Cloning, Sequencing, and Expression of a Novel Multidomain Mannanase Gene from Thermoanaerobacterium polysaccharolyticum. J. Bacteriol. 181: 1643-1651 [Abstract] [Full Text]  
  • Navarre, W. W., Schneewind, O. (1999). Surface Proteins of Gram-Positive Bacteria and Mechanisms of Their Targeting to the Cell Wall Envelope. Microbiol. Mol. Biol. Rev. 63: 174-229 [Abstract] [Full Text]  
  • Sára, M., Egelseer, E. M., Dekitsch, C., Sleytr, U. B. (1998). Identification of Two Binding Domains, One for Peptidoglycan and Another for a Secondary Cell Wall Polymer, on the N-Terminal Part of the S-Layer Protein SbsB from Bacillus stearothermophilus PV72/p2. J. Bacteriol. 180: 6780-6783 [Abstract] [Full Text]  
  • Sára, M., Dekitsch, C., Mayer, H. F., Egelseer, E. M., Sleytr, U. B. (1998). Influence of the Secondary Cell Wall Polymer on the Reassembly, Recrystallization, and Stability Properties of the S-Layer Protein from Bacillus stearothermophilus PV72/p2. J. Bacteriol. 180: 4146-4153 [Abstract] [Full Text]  
  • Egelseer, E. M., Leitner, K., Jarosch, M., Hotzy, C., Zayni, S., Sleytr, U. B., Sára, M. (1998). The S-Layer Proteins of Two Bacillus stearothermophilus Wild-Type Strains Are Bound via Their N-Terminal Region to a Secondary Cell Wall Polymer of Identical Chemical Composition. J. Bacteriol. 180: 1488-1495 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»