This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gomez-Gomez, J. M.
Right arrow Articles by Blazquez, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gomez-Gomez, J. M.
Right arrow Articles by Blazquez, J.

 Previous Article  |  Next Article 

J. Bacteriol., 06 1996, 3331-3334, Vol 178, No. 11
Copyright © 1996, American Society for Microbiology

Cyclic AMP receptor protein positively controls gyrA transcription and alters DNA topology after nutritional upshift in Escherichia coli

JM Gomez-Gomez, F Baquero and J Blazquez
Servico de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain.

The expression of a transcriptional gyrA-lacZ gene fusion throughout the Escherichia coli growth cycle and the effect that mutation delta crp39 had on this expression were studied. The data obtained indicate that the expression of gyrA is growth phase dependent and under the positive control of the cyclic AMP receptor protein (CRP). Complementation analysis of gyrA-lacZ expression with wild-type CRP or variant CRP pc (with a T-to-A mutation at position 158) in a CRP- deficient background suggests that this CRP action is mediated by a class I or class II CRP-dependent promoter(s). Our results also indicate that CRP may be involved in the modulation of DNA topology in the transition from the lag period to the exponential phase of growth.


This article has been cited by other articles:

  • Bradley, M. D., Beach, M. B., de Koning, A. P. J., Pratt, T. S., Osuna, R. (2007). Effects of Fis on Escherichia coli gene expression during different growth stages. Microbiology 153: 2922-2940 [Abstract] [Full Text]  
  • Starcic, M., Zgur-Bertok, D., Jordi, B. J. A. M., Wosten, M. M. S. M., Gaastra, W., van Putten, J. P. M. (2003). The Cyclic AMP-Cyclic AMP Receptor Protein Complex Regulates Activity of the traJ Promoter of the Escherichia coli Conjugative Plasmid pRK100. J. Bacteriol. 185: 1616-1623 [Abstract] [Full Text]  
  • Reyes-Dominguez, Y., Contreras-Ferrat, G., Ramirez-Santos, J., Membrillo-Hernandez, J., Gomez-Eichelmann, M. C. (2003). Plasmid DNA Supercoiling and Gyrase Activity in Escherichia coli Wild-Type and rpoS Stationary-Phase Cells. J. Bacteriol. 185: 1097-1100 [Abstract] [Full Text]  
  • Kuhar, I. (1999). Transcription Regulation of the Colicin K cka Gene Reveals Induction of Colicin Synthesis by Differential Responses to Environmental Signals. J. Bacteriol. 181: 7373-7380 [Abstract] [Full Text]  
  • Hu, S.-T., Wang, H.-C., Lei, G.-S., Wang, S.-H. (1998). Negative Regulation of IS2 Transposition by the Cyclic AMP (cAMP)-cAMP Receptor Protein Complex. J. Bacteriol. 180: 2682-2688 [Abstract] [Full Text]  
  • Nakanishi, A., Oshida, T., Matsushita, T., Imajoh-Ohmi, S., Ohnuki, T. (1998). Identification of DNA Gyrase Inhibitor (GyrI) in Escherichia coli. J. Biol. Chem. 273: 1933-1938 [Abstract] [Full Text]  
  • Nasser, W., Schneider, R., Travers, A., Muskhelishvili, G. (2001). CRP Modulates fis Transcription by Alternate Formation of Activating and Repressing Nucleoprotein Complexes. J. Biol. Chem. 276: 17878-17886 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»