This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khudyakov, I.
Right arrow Articles by Wolk, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khudyakov, I.
Right arrow Articles by Wolk, C. P.

 Previous Article  |  Next Article 

J. Bacteriol., 06 1996, 3572-3577, Vol 178, No. 12
Copyright © 1996, American Society for Microbiology

Evidence that the hanA gene coding for HU protein is essential for heterocyst differentiation in, and cyanophage A-4(L) sensitivity of, Anabaena sp. strain PCC 7120

I Khudyakov and CP Wolk
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA.

The highly pleiotropic, transposon-generated mutant AB22 of Anabaena sp. strain PCC 7120 exhibits slow growth, altered pigmentation, cellular fragility, resistance to phage A-4(L), and the inability to differentiate heterocysts. Reconstruction of the transposon mutation in the wild-type strain reproduced the phenotype of the original mutant. Sequencing of the flanking DNA showed that the transposon had inserted at the beginning of a gene, which we call hanA, that encodes Anabaena HU protein (R. Nagaraja and R. Haselkorn, Biochimie 76:1082-1089, 1994). Mapping of the transposon insertion by pulsed-field gel electrophoresis showed that hanA is located at ca. 4.76 Mb on the physical map of the chromosome and is transcribed clockwise. Repeated subculturing of AB22 resulted in improved growth and loss of filament fragmentation, presumably because of one or more compensatory mutations; however, the mutant retained its A-4(L)r Het- phenotype. The mutation in strain AB22 could be complemented by a fragment of wild- type DNA bearing hanA as its only open reading frame.


This article has been cited by other articles:

  • Melnikov, O., Zaritsky, A., Zarka, A., Boussiba, S., Malchin, N., Yagil, E., Kolot, M. (2009). Site-Specific Recombination in the Cyanobacterium Anabaena sp. Strain PCC 7120 Catalyzed by the Integrase of Coliphage HK022. J. Bacteriol. 191: 4458-4464 [Abstract] [Full Text]  
  • Khudyakov, I. Y., Golden, J. W. (2004). Different functions of HetR, a master regulator of heterocyst differentiation in Anabaena sp. PCC 7120, can be separated by mutation. Proc. Natl. Acad. Sci. USA 101: 16040-16045 [Abstract] [Full Text]  
  • El-Shehawy, R., Lugomela, C., Ernst, A., Bergman, B. (2003). Diurnal expression of hetR and diazocyte development in the filamentous non-heterocystous cyanobacterium Trichodesmium erythraeum. Microbiology 149: 1139-1146 [Abstract] [Full Text]  
  • Koksharova, O. A., Wolk, C. P. (2002). Novel DNA-Binding Proteins in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 3931-3940 [Abstract] [Full Text]  
  • Meeks, J. C., Elhai, J. (2002). Regulation of Cellular Differentiation in Filamentous Cyanobacteria in Free-Living and Plant-Associated Symbiotic Growth States. Microbiol. Mol. Biol. Rev. 66: 94-121 [Abstract] [Full Text]  
  • Zhu, J., Jager, K., Black, T., Zarka, K., Koksharova, O., Wolk, C. P. (2001). HcwA, an Autolysin, Is Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 183: 6841-6851 [Abstract] [Full Text]  
  • Khudyakov, I. Y., Golden, J. W. (2001). Identification and Inactivation of Three Group 2 Sigma Factor Genes in Anabaena sp. Strain PCC 7120. J. Bacteriol. 183: 6667-6675 [Abstract] [Full Text]  
  • Wong, F. C. Y., Meeks, J. C. (2001). The hetF Gene Product Is Essential to Heterocyst Differentiation and Affects HetR Function in the Cyanobacterium Nostoc punctiforme. J. Bacteriol. 183: 2654-2661 [Abstract] [Full Text]  
  • Herrero, A., Muro-Pastor, A. M., Flores, E. (2001). Nitrogen Control in Cyanobacteria. J. Bacteriol. 183: 411-425 [Full Text]  
  • Thiel, T., Pratte, B. (2001). Effect on Heterocyst Differentiation of Nitrogen Fixation in Vegetative Cells of the Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 183: 280-286 [Abstract] [Full Text]  
  • Zhu, J., Kong, R., Wolk, C. P. (1998). Regulation of hepA of Anabaena sp. Strain PCC 7120 by Elements 5' from the Gene and by hepK. J. Bacteriol. 180: 4233-4242 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»