This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hassett, D. J.
Right arrow Articles by Vasil, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hassett, D. J.
Right arrow Articles by Vasil, M. L.

 Previous Article  |  Next Article 

J. Bacteriol., Jul 1996, 3996-4003, Vol 178, No. 14
Copyright © 1996, American Society for Microbiology

Ferric uptake regulator (Fur) mutants of Pseudomonas aeruginosa demonstrate defective siderophore-mediated iron uptake, altered aerobic growth, and decreased superoxide dismutase and catalase activities

DJ Hassett, PA Sokol, ML Howell, JF Ma, HT Schweizer, U Ochsner and ML Vasil
Department of Molecular Genetics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524, USA.

Pseudomonas aeruginosa is considered a strict aerobe that possesses several enzymes important in the disposal of toxic oxygen reduction products including iron- and manganese-cofactored superoxide dismutase and catalase. At present, the nature of the regulation of these enzymes in P. aeruginosa Is not understood. To address these issues, we used two mutants called A4 and C6 which express altered Fur (named for ferric uptake regulation) proteins and constitutively produce the siderophores pyochelin and pyoverdin. Both mutants required a significant lag phase prior to log-phase aerobic growth, but this lag was not as apparent when the organisms were grown under microaerobic conditions. The addition of iron salts to mutant A4 and, to a greater extent, C6 cultures allowed for an increased growth rate under both conditions relative to that of bacteria without added iron. Increased manganese superoxide dismutase (Mn-SOD) and decreased catalase activities were also apparent in the mutants, although the second catalase, KatB, was detected in cell extracts of each fur mutant. Iron deprivation by the addition of the iron chelator 2,2'-dipyridyl to wild- type bacteria produced an increase in Mn-SOD activity and a decrease in total catalase activity, similar to the fur mutant phenotype. Purified wild-type Fur bound more avidly than mutant Fur to a PCR product containing two palindromic 19-bp "iron box" regions controlling expression of an operon containing the sodA gene that encodes Mn-SOD. All mutants were defective in both ferripyochelin- and ferripyoverdin- mediated iron uptake. Two mutants of strain PAO1, defective in pyoverdin but not pyochelin biosynthesis, produced increased Mn-SOD activity. Sensitivity to both the redox-cycling agent paraquat and hydrogen peroxide was greater in each mutant than in the wild-type strain. In summary, the results indicate that mutations in the P. aeruginosa fur locus affect aerobic growth and SOD and catalase activities in P. aeruginosa. We postulate that reduced siderophore- mediated iron uptake, especially that by pyoverdin, may be one possible mechanism contributing to such effect.


This article has been cited by other articles:

  • da Silva Neto, J. F., Braz, V. S., Italiani, V. C. S., Marques, M. V. (2009). Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus. Nucleic Acids Res 37: 4812-4825 [Abstract] [Full Text]  
  • Carpenter, B. M., Whitmire, J. M., Merrell, D. S. (2009). This Is Not Your Mother's Repressor: the Complex Role of Fur in Pathogenesis. Infect. Immun. 77: 2590-2601 [Full Text]  
  • Patrauchan, M. A., Sarkisova, S. A., Franklin, M. J. (2007). Strain-specific proteome responses of Pseudomonas aeruginosa to biofilm-associated growth and to calcium. Microbiology 153: 3838-3851 [Abstract] [Full Text]  
  • Bender, K. S., Yen, H.-C. B., Hemme, C. L., Yang, Z., He, Z., He, Q., Zhou, J., Huang, K. H., Alm, E. J., Hazen, T. C., Arkin, A. P., Wall, J. D. (2007). Analysis of a Ferric Uptake Regulator (Fur) Mutant of Desulfovibrio vulgaris Hildenborough. Appl. Environ. Microbiol. 73: 5389-5400 [Abstract] [Full Text]  
  • Kitphati, W., Ngok-ngam, P., Suwanmaneerat, S., Sukchawalit, R., Mongkolsuk, S. (2007). Agrobacterium tumefaciens fur Has Important Physiological Roles in Iron and Manganese Homeostasis, the Oxidative Stress Response, and Full Virulence. Appl. Environ. Microbiol. 73: 4760-4768 [Abstract] [Full Text]  
  • Wang, N., Lu, S.-E., Records, A. R., Gross, D. C. (2006). Characterization of the Transcriptional Activators SalA and SyrF, Which Are Required for Syringomycin and Syringopeptin Production by Pseudomonas syringae pv. syringae. J. Bacteriol. 188: 3290-3298 [Abstract] [Full Text]  
  • Chao, T.-C., Buhrmester, J., Hansmeier, N., Puhler, A., Weidner, S. (2005). Role of the Regulatory Gene rirA in the Transcriptional Response of Sinorhizobium meliloti to Iron Limitation. Appl. Environ. Microbiol. 71: 5969-5982 [Abstract] [Full Text]  
  • Salunkhe, P., Topfer, T., Buer, J., Tummler, B. (2005). Genome-Wide Transcriptional Profiling of the Steady-State Response of Pseudomonas aeruginosa to Hydrogen Peroxide. J. Bacteriol. 187: 2565-2572 [Abstract] [Full Text]  
  • Parker, D., Kennan, R. M., Myers, G. S., Paulsen, I. T., Rood, J. I. (2005). Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets. J. Bacteriol. 187: 366-375 [Abstract] [Full Text]  
  • Wan, X.-F., VerBerkmoes, N. C., McCue, L. A., Stanek, D., Connelly, H., Hauser, L. J., Wu, L., Liu, X., Yan, T., Leaphart, A., Hettich, R. L., Zhou, J., Thompson, D. K. (2004). Transcriptomic and Proteomic Characterization of the Fur Modulon in the Metal-Reducing Bacterium Shewanella oneidensis. J. Bacteriol. 186: 8385-8400 [Abstract] [Full Text]  
  • Friedman, Y. E., O'Brian, M. R. (2004). The Ferric Uptake Regulator (Fur) Protein from Bradyrhizobium japonicum Is an Iron-responsive Transcriptional Repressor in Vitro. J. Biol. Chem. 279: 32100-32105 [Abstract] [Full Text]  
  • Chao, T.-C., Becker, A., Buhrmester, J., Puhler, A., Weidner, S. (2004). The Sinorhizobium meliloti fur Gene Regulates, with Dependence on Mn(II), Transcription of the sitABCD Operon, Encoding a Metal-Type Transporter. J. Bacteriol. 186: 3609-3620 [Abstract] [Full Text]  
  • Benson, H. P., LeVier, K., Guerinot, M. L. (2004). A Dominant-Negative fur Mutation in Bradyrhizobium japonicum. J. Bacteriol. 186: 1409-1414 [Abstract] [Full Text]  
  • Harris, A. G., Hinds, F. E., Beckhouse, A. G., Kolesnikow, T., Hazell, S. L. (2002). Resistance to hydrogen peroxide in Helicobacter pylori: role of catalase (KatA) and Fur, and functional analysis of a novel gene product designated 'KatA-associated protein', KapA (HP0874). Microbiology 148: 3813-3825 [Abstract] [Full Text]  
  • Ricci, S., Janulczyk, R., Bjorck, L. (2002). The Regulator PerR Is Involved in Oxidative Stress Response and Iron Homeostasis and Is Necessary for Full Virulence of Streptococcus pyogenes. Infect. Immun. 70: 4968-4976 [Abstract] [Full Text]  
  • Lewin, A. C., Doughty, P. A., Flegg, L., Moore, G. R., Spiro, S. (2002). The ferric uptake regulator of Pseudomonas aeruginosa has no essential cysteine residues and does not contain a structural zinc ion. Microbiology 148: 2449-2456 [Abstract] [Full Text]  
  • Spatafora, G., Van Hoeven, N., Wagner, K., Fives-Taylor, P. (2002). Evidence that ORF3 at the Streptococcus parasanguis fimA locus encodes a thiol-specific antioxidant. Microbiology 148: 755-762 [Abstract] [Full Text]  
  • Thompson, D. K., Beliaev, A. S., Giometti, C. S., Tollaksen, S. L., Khare, T., Lies, D. P., Nealson, K. H., Lim, H., Yates, J. III, Brandt, C. C., Tiedje, J. M., Zhou, J. (2002). Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress. Appl. Environ. Microbiol. 68: 881-892 [Abstract] [Full Text]  
  • Britigan, B. E., Miller, R. A., Hassett, D. J., Pfaller, M. A., McCormick, M. L., Rasmussen, G. T. (2001). Antioxidant Enzyme Expression in Clinical Isolates of Pseudomonas aeruginosa: Identification of an Atypical Form of Manganese Superoxide Dismutase. Infect. Immun. 69: 7396-7401 [Abstract] [Full Text]  
  • Bollinger, N., Hassett, D. J., Iglewski, B. H., Costerton, J. W., McDermott, T. R. (2001). Gene Expression in Pseudomonas aeruginosa: Evidence of Iron Override Effects on Quorum Sensing and Biofilm-Specific Gene Regulation. J. Bacteriol. 183: 1990-1996 [Abstract] [Full Text]  
  • Frederick, J. R., Elkins, J. G., Bollinger, N., Hassett, D. J., McDermott, T. R. (2001). Factors Affecting Catalase Expression in Pseudomonas aeruginosa Biofilms and Planktonic Cells. Appl. Environ. Microbiol. 67: 1375-1379 [Abstract] [Full Text]  
  • Bereswill, S., Greiner, S., van Vliet, A. H. M., Waidner, B., Fassbinder, F., Schiltz, E., Kusters, J. G., Kist, M. (2000). Regulation of Ferritin-Mediated Cytoplasmic Iron Storage by the Ferric Uptake Regulator Homolog (Fur) of Helicobacter pylori. J. Bacteriol. 182: 5948-5953 [Abstract] [Full Text]  
  • Baysse, C., De Vos, D., Naudet, Y., Vandermonde, A., Ochsner, U., Meyer, J.-M., Budzikiewicz, H., Schäfer, M., Fuchs, R., Cornelis, P. (2000). Vanadium interferes with siderophore-mediated iron uptake in Pseudomonas aeruginosa. Microbiology 146: 2425-2434 [Abstract] [Full Text]  
  • Ma, J.-F., Ochsner, U. A., Klotz, M. G., Nanayakkara, V. K., Howell, M. L., Johnson, Z., Posey, J. E., Vasil, M. L., Monaco, J. J., Hassett, D. J. (1999). Bacterioferritin A Modulates Catalase A (KatA) Activity and Resistance to Hydrogen Peroxide in Pseudomonas aeruginosa. J. Bacteriol. 181: 3730-3742 [Abstract] [Full Text]  
  • van Vliet, A. H. M., Wooldridge, K. G., Ketley, J. M. (1998). Iron-Responsive Gene Regulation in a Campylobacter jejuni fur Mutant. J. Bacteriol. 180: 5291-5298 [Abstract] [Full Text]  
  • Zhao, Q., Li, X.-Z., Mistry, A., Srikumar, R., Zhang, L., Lomovskaya, O., Poole, K. (1998). Influence of the TonB Energy-Coupling Protein on Efflux-Mediated Multidrug Resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 42: 2225-2231 [Abstract] [Full Text]  
  • Ma, J.-F., Hager, P. W., Howell, M. L., Phibbs, P. V., Hassett, D. J. (1998). Cloning and Characterization of the Pseudomonas aeruginosa zwf Gene Encoding Glucose-6-Phosphate Dehydrogenase, an Enzyme Important in Resistance to Methyl Viologen (Paraquat). J. Bacteriol. 180: 1741-1749 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»