This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, C. E.
Right arrow Articles by Sparling, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, C. E.
Right arrow Articles by Sparling, P. F.

 Previous Article  |  Next Article 

J. Bacteriol., 07 1996, 4224-4232, Vol 178, No. 14
Copyright © 1996, American Society for Microbiology

Isolation and analysis of a fur mutant of Neisseria gonorrhoeae

CE Thomas and PF Sparling
Department of Microbiology, School of Medicine, University of North Carolina at Chapel Hill, 27599, USA.

The pathogenic Neisseria spp. produce a number of iron-regulated gene products that are thought to be important in virulence. Iron-responsive regulation of these gene products has been attributed to the presence in Neisseria spp. of the Fur (ferric uptake regulation) protein. Evidence for the role of Fur in neisserial iron regulation has been indirect because of the inability to make fur null mutations. To circumvent this problem, we used manganese selection to isolate missense mutations of Neisseria gonorrhoeae fur. We show that a mutation in gonococcal fur resulted in reduced modulation of expression of four well-studied iron-repressed genes and affected the iron regulation of a broad range of other genes as judged by two-dimensional polyacrylamide gel electrophoresis (PAGE). All 15 of the iron-repressed spots observed by two-dimensional PAGE were at least partially derepressed in the fur mutant, and 17 of the 45 iron-induced spots were affected by the fur mutation. Thus, Fur plays a central role in regulation of iron-repressed gonococcal genes and appears to be involved in regulation of many iron-induced genes. The size and complexity of the iron regulons in N. gonorrhoeae are much greater than previously recognized.


This article has been cited by other articles:

  • Seib, K. L., Wu, H.-J., Kidd, S. P., Apicella, M. A., Jennings, M. P., McEwan, A. G. (2006). Defenses against Oxidative Stress in Neisseria gonorrhoeae: a System Tailored for a Challenging Environment. Microbiol. Mol. Biol. Rev. 70: 344-361 [Abstract] [Full Text]  
  • Haraszthy, V. I., Jordan, S. F., Zambon, J. J. (2006). Identification of Fur-regulated genes in Actinobacillus actinomycetemcomitans.. Microbiology 152: 787-796 [Abstract] [Full Text]  
  • Ducey, T. F., Carson, M. B., Orvis, J., Stintzi, A. P., Dyer, D. W. (2005). Identification of the Iron-Responsive Genes of Neisseria gonorrhoeae by Microarray Analysis in Defined Medium. J. Bacteriol. 187: 4865-4874 [Abstract] [Full Text]  
  • Harvie, D. R., Vilchez, S., Steggles, J. R., Ellar, D. J. (2005). Bacillus cereus Fur regulates iron metabolism and is required for full virulence. Microbiology 151: 569-577 [Abstract] [Full Text]  
  • Parker, D., Kennan, R. M., Myers, G. S., Paulsen, I. T., Rood, J. I. (2005). Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets. J. Bacteriol. 187: 366-375 [Abstract] [Full Text]  
  • Wan, X.-F., VerBerkmoes, N. C., McCue, L. A., Stanek, D., Connelly, H., Hauser, L. J., Wu, L., Liu, X., Yan, T., Leaphart, A., Hettich, R. L., Zhou, J., Thompson, D. K. (2004). Transcriptomic and Proteomic Characterization of the Fur Modulon in the Metal-Reducing Bacterium Shewanella oneidensis. J. Bacteriol. 186: 8385-8400 [Abstract] [Full Text]  
  • Alexander, H. L., Rasmussen, A. W., Stojiljkovic, I. (2004). Identification of Neisseria meningitidis Genetic Loci Involved in the Modulation of Phase Variation Frequencies. Infect. Immun. 72: 6743-6747 [Abstract] [Full Text]  
  • Chao, T.-C., Becker, A., Buhrmester, J., Puhler, A., Weidner, S. (2004). The Sinorhizobium meliloti fur Gene Regulates, with Dependence on Mn(II), Transcription of the sitABCD Operon, Encoding a Metal-Type Transporter. J. Bacteriol. 186: 3609-3620 [Abstract] [Full Text]  
  • Perkins-Balding, D., Ratliff-Griffin, M., Stojiljkovic, I. (2004). Iron Transport Systems in Neisseria meningitidis. Microbiol. Mol. Biol. Rev. 68: 154-171 [Abstract] [Full Text]  
  • Delany, I., Ieva, R., Alaimo, C., Rappuoli, R., Scarlato, V. (2003). The Iron-Responsive Regulator Fur Is Transcriptionally Autoregulated and Not Essential in Neisseria meningitidis. J. Bacteriol. 185: 6032-6041 [Abstract] [Full Text]  
  • Grifantini, R., Sebastian, S., Frigimelica, E., Draghi, M., Bartolini, E., Muzzi, A., Rappuoli, R., Grandi, G., Genco, C. A. (2003). Identification of iron-activated and -repressed Fur-dependent genes by transcriptome analysis of Neisseria meningitidis group B. Proc. Natl. Acad. Sci. USA 100: 9542-9547 [Abstract] [Full Text]  
  • Sebastian, S., Agarwal, S., Murphy, J. R., Genco, C. A. (2002). The Gonococcal Fur Regulon: Identification of Additional Genes Involved in Major Catabolic, Recombination, and Secretory Pathways. J. Bacteriol. 184: 3965-3974 [Abstract] [Full Text]  
  • Jerse, A. E., Crow, E. T., Bordner, A. N., Rahman, I., Cornelissen, C. N., Moench, T. R., Mehrazar, K. (2002). Growth of Neisseria gonorrhoeae in the Female Mouse Genital Tract Does Not Require the Gonococcal Transferrin or Hemoglobin Receptors and May Be Enhanced by Commensal Lactobacilli. Infect. Immun. 70: 2549-2558 [Abstract] [Full Text]  
  • Thompson, D. K., Beliaev, A. S., Giometti, C. S., Tollaksen, S. L., Khare, T., Lies, D. P., Nealson, K. H., Lim, H., Yates, J. III, Brandt, C. C., Tiedje, J. M., Zhou, J. (2002). Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress. Appl. Environ. Microbiol. 68: 881-892 [Abstract] [Full Text]  
  • Panina, E. M., Mironov, A. A., Gelfand, M. S. (2001). Comparative analysis of FUR regulons in gamma-proteobacteria. Nucleic Acids Res 29: 5195-5206 [Abstract] [Full Text]  
  • Turner, P. C., Thomas, C. E., Stojiljkovic, I., Elkins, C., Kizel, G., Ala’Aldeen, D. A. A., Sparling, P. F. (2001). Neisserial TonB-dependent outer-membrane proteins: detection, regulation and distribution of three putative candidates identified from the genome sequences. Microbiology 147: 1277-1290 [Abstract] [Full Text]  
  • Escolar, L., Pérez-Martín, J., de Lorenzo, V. (1999). Opening the Iron Box: Transcriptional Metalloregulation by the Fur Protein. J. Bacteriol. 181: 6223-6229 [Full Text]  
  • Turner, P. C., Thomas, C. E., Elkins, C., Clary, S., Sparling, P. F. (1998). Neisseria gonorrhoeae Heme Biosynthetic Mutants Utilize Heme and Hemoglobin as a Heme Source but Fail To Grow within Epithelial Cells. Infect. Immun. 66: 5215-5223 [Abstract] [Full Text]  
  • van Vliet, A. H. M., Wooldridge, K. G., Ketley, J. M. (1998). Iron-Responsive Gene Regulation in a Campylobacter jejuni fur Mutant. J. Bacteriol. 180: 5291-5298 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»