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J. Bacteriol., 08 1996, 4731-4733, Vol 178, No. 15
Copyright © 1996, American Society for Microbiology

Conversion of NfsB, a minor Escherichia coli nitroreductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, by a single amino acid substitution

S Zenno, H Koike, M Tanokura and K Saigo
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.

NfsB is an oxygen-insensitive nitroreductase of Escherichia coli with significant amino acid sequence homology to the major flavin reductase (FRase I) of Vibrio fischeri. Here, we show that NfsB is convertible to an FRase I-like flavin reductase three times as active as the authentic FRase I by a single amino acid substitution in the least-conserved region.

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