This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morbidoni, H. R.
Right arrow Articles by Cronan, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morbidoni, H. R.
Right arrow Articles by Cronan, J. E., Jr

 Previous Article  |  Next Article 

J. Bacteriol., Aug 1996, 4794-4800, Vol 178, No. 16
Copyright © 1996, American Society for Microbiology

Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes

HR Morbidoni, D de Mendoza and JE Cronan Jr
Department of Microbiology, University of Illinois, Urbana 61801, USA.

A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme A-acyl carrier protein transacylase (fabD), 3-ketoacyl-acyl carrier protein reductase (fabG), and the N-terminal 14 amino acid residues of acyl carrier protein (acpP). Also present is a sequence that encodes a homolog of E. coli plsX, a gene that plays a poorly understood role in phospholipid synthesis. The B. subtilis plsX gene weakly complemented an E. coli plsX mutant. The order of genes in the cluster is plsX fabD fabG acpP, the same order found in E. coli, except that in E. coli the fabH gene lies between plsX and fabD. The absence of fabH in the B. subtilis cluster is consistent with the different fatty acid compositions of the two organisms. The amino acid sequence of B. subtilis acyl carrier protein was obtained by sequencing the purified protein, and the sequence obtained strongly resembled that of E. coli acyl carrier protein, except that most of the protein retained the initiating methionine residue. The B. subtilis fab cluster was mapped to the 135 to 145 degrees region of the chromosome.


This article has been cited by other articles:

  • McAllister, K. A., Peery, R. B., Zhao, G. (2006). Acyl Carrier Protein Synthases from Gram-Negative, Gram-Positive, and Atypical Bacterial Species: Biochemical and Structural Properties and Physiological Implications. J. Bacteriol. 188: 4737-4748 [Abstract] [Full Text]  
  • Seidle, H., Rangaswamy, V., Couch, R., Bender, C. L., Parry, R. J. (2004). Characterization of Cfa1, a Monofunctional Acyl Carrier Protein Involved in the Biosynthesis of the Phytotoxin Coronatine. J. Bacteriol. 186: 2499-2503 [Abstract] [Full Text]  
  • Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K., Ashikaga, S., Aymerich, S., Bessieres, P., Boland, F., Brignell, S. C., Bron, S., Bunai, K., Chapuis, J., Christiansen, L. C., Danchin, A., Debarbouille, M., Dervyn, E., Deuerling, E., Devine, K., Devine, S. K., Dreesen, O., Errington, J., Fillinger, S., Foster, S. J., Fujita, Y., Galizzi, A., Gardan, R., Eschevins, C., Fukushima, T., Haga, K., Harwood, C. R., Hecker, M., Hosoya, D., Hullo, M. F., Kakeshita, H., Karamata, D., Kasahara, Y., Kawamura, F., Koga, K., Koski, P., Kuwana, R., Imamura, D., Ishimaru, M., Ishikawa, S., Ishio, I., Le Coq, D., Masson, A., Mauel, C., Meima, R., Mellado, R. P., Moir, A., Moriya, S., Nagakawa, E., Nanamiya, H., Nakai, S., Nygaard, P., Ogura, M., Ohanan, T., O'Reilly, M., O'Rourke, M., Pragai, Z., Pooley, H. M., Rapoport, G., Rawlins, J. P., Rivas, L. A., Rivolta, C., Sadaie, A., Sadaie, Y., Sarvas, M., Sato, T., Saxild, H. H., Scanlan, E., Schumann, W., Seegers, J. F. M. L., Sekiguchi, J., Sekowska, A., Seror, S. J., Simon, M., Stragier, P., Studer, R., Takamatsu, H., Tanaka, T., Takeuchi, M., Thomaides, H. B., Vagner, V., van Dijl, J. M., Watabe, K., Wipat, A., Yamamoto, H., Yamamoto, M., Yamamoto, Y., Yamane, K., Yata, K., Yoshida, K., Yoshikawa, H., Zuber, U., Ogasawara, N. (2003). Essential Bacillussubtilis genes. Proc. Natl. Acad. Sci. USA 100: 4678-4683 [Abstract] [Full Text]  
  • Tsuge, K., Akiyama, T., Shoda, M. (2001). Cloning, Sequencing, and Characterization of the Iturin A Operon. J. Bacteriol. 183: 6265-6273 [Abstract] [Full Text]  
  • Kiatpapan, P., Kobayashi, H., Sakaguchi, M., Ono, H., Yamashita, M., Kaneko, Y., Murooka, Y. (2001). Molecular Characterization of Lactobacillus plantarum Genes for {beta}-Ketoacyl-Acyl Carrier Protein Synthase III (fabH) and Acetyl Coenzyme A Carboxylase (accBCDA), Which Are Essential for Fatty Acid Biosynthesis. Appl. Environ. Microbiol. 67: 426-433 [Abstract] [Full Text]  
  • Debabov, D. V., Kiriukhin, M. Y., Neuhaus, F. C. (2000). Biosynthesis of Lipoteichoic Acid in Lactobacillus rhamnosus: Role of DltD in D-Alanylation. J. Bacteriol. 182: 2855-2864 [Abstract] [Full Text]  
  • Kutchma, A. J., Hoang, T. T., Schweizer, H. P. (1999). Characterization of a Pseudomonas aeruginosa Fatty Acid Biosynthetic Gene Cluster: Purification of Acyl Carrier Protein (ACP) and Malonyl-Coenzyme A:ACP Transacylase (FabD). J. Bacteriol. 181: 5498-5504 [Abstract] [Full Text]  
  • Spellerberg, B., Pohl, B., Haase, G., Martin, S., Weber-Heynemann, J., Lütticken, R. (1999). Identification of Genetic Determinants for the Hemolytic Activity of Streptococcus agalactiae by ISS1 Transposition. J. Bacteriol. 181: 3212-3219 [Abstract] [Full Text]  
  • Follens, A., Veiga-da-Cunha, M., Merckx, R., van Schaftingen, E., van Eldere, J. (1999). acs1 of Haemophilus influenzae Type a Capsulation Locus Region II Encodes a Bifunctional Ribulose 5-Phosphate Reductase- CDP-Ribitol Pyrophosphorylase. J. Bacteriol. 181: 2001-2007 [Abstract] [Full Text]  
  • McCool, G. J., Cannon, M. C. (1999). Polyhydroxyalkanoate Inclusion Body-Associated Proteins and Coding Region in Bacillus megaterium. J. Bacteriol. 181: 585-592 [Abstract] [Full Text]  
  • Han, L., Lobo, S., Reynolds, K. A. (1998). Characterization of beta -Ketoacyl-Acyl Carrier Protein Synthase III from Streptomyces glaucescens and Its Role in Initiation of Fatty Acid Biosynthesis. J. Bacteriol. 180: 4481-4486 [Abstract] [Full Text]  
  • Zhang, Y., Cronan, J. E. Jr. (1998). Transcriptional Analysis of Essential Genes of the Escherichia coli Fatty Acid Biosynthesis Gene Cluster by Functional Replacement with the Analogous Salmonella typhimurium Gene Cluster. J. Bacteriol. 180: 3295-3303 [Abstract] [Full Text]  
  • McAllister, K. A., Peery, R. B., Meier, T. I., Fischl, A. S., Zhao, G. (2000). Biochemical and Molecular Analyses of the Streptococcus pneumoniae Acyl Carrier Protein Synthase, an Enzyme Essential for Fatty Acid Biosynthesis. J. Biol. Chem. 275: 30864-30872 [Abstract] [Full Text]  
  • Mootz, H. D., Finking, R., Marahiel, M. A. (2001). 4'-Phosphopantetheine Transfer in Primary and Secondary Metabolism of Bacillus subtilis. J. Biol. Chem. 276: 37289-37298 [Abstract] [Full Text]  
  • Kremer, L., Nampoothiri, K. M., Lesjean, S., Dover, L. G., Graham, S., Betts, J., Brennan, P. J., Minnikin, D. E., Locht, C., Besra, G. S. (2001). Biochemical Characterization of Acyl Carrier Protein (AcpM) and Malonyl-CoA:AcpM Transacylase (mtFabD), Two Major Components of Mycobacterium tuberculosis Fatty Acid Synthase II. J. Biol. Chem. 276: 27967-27974 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»