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J. Bacteriol., Sep 1996, 5537-5539, Vol 178, No. 18
Copyright © 1996, American Society for Microbiology

In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni)

M Salema, I Capucho, B Poolman, MV San Romao and MC Dias
Instituto de Technologia Quimica e Biologica, U.N.L., Oeiras, Portugal.

The mechanism of metabolic energy generation by malolactic fermentation was studied with artificial membrane vesicles of Leuconostoc oenos (Oenococcus oeni). (Note that although L. oenos was recently reclassified as O. oeni [L. M. T. Dicks, F. Dellaglio, and M. D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995], the old designation was kept in the present work.) Purified malolactic enzyme was entrapped in artificial membrane vesicles prepared from L. oenos cells able to transport L-malate. We show that the in vitro reconstituted system, including an electrogenic L-malate carrier and the decarboxylating malolactic enzyme, generated a proton motive force that was able to drive intravesicular accumulation of leucine.

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