This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petersen, L.
Right arrow Articles by Downs, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petersen, L.
Right arrow Articles by Downs, D. M.

 Previous Article  |  Next Article 

J. Bacteriol., Oct 1996, 5676-5682, Vol 178, No. 19
Copyright © 1996, American Society for Microbiology

Mutations in apbC (mrp) prevent function of the alternative pyrimidine biosynthetic pathway in Salmonella typhimurium

L Petersen and DM Downs
Department of Bacteriology, University of Wisconsin--Madison, 53706, USA.

The alternative pyrimidine biosynthetic (APB) pathway can synthesize the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine in Salmonella typhimurium independently of de novo purine biosynthesis. When mutants defective in function of the APB pathway were isolated, the predominant class (40%) were defective in a single locus we have designated apbC. Mutations in apbC block function of the APB pathway since they prevent growth of a purF mutant in the absence of thiamine. Lesions in apbC also cause a thiamine auxotrophy in strains proficient in purine biosynthesis when fructose is provided as the sole carbon and energy source. Results presented here are consistent with ApbC being involved in the conversion of aminoimidazole ribonucleotide to HMP, and we suggest that ApbC performs a redundant step in thiamine synthesis. Sequence analysis demonstrated that apbC mutations were alleles of mrp, a locus previously reported in Escherichia coli as a metG-related protein. We propose that this locus in S. typhimurium be designated apbC to reflect its involvement in thiamine synthesis.


This article has been cited by other articles:

  • Boyd, J. M., Drevland, R. M., Downs, D. M., Graham, D. E. (2009). Archaeal ApbC/Nbp35 Homologs Function as Iron-Sulfur Cluster Carrier Proteins. J. Bacteriol. 191: 1490-1497 [Abstract] [Full Text]  
  • Boyd, J. M., Sondelski, J. L., Downs, D. M. (2009). Bacterial ApbC Protein Has Two Biochemical Activities That Are Required for in Vivo Function. J. Biol. Chem. 284: 110-118 [Abstract] [Full Text]  
  • Boyd, J. M., Lewis, J. A., Escalante-Semerena, J. C., Downs, D. M. (2008). Salmonella enterica Requires ApbC Function for Growth on Tricarballylate: Evidence of Functional Redundancy between ApbC and IscU. J. Bacteriol. 190: 4596-4602 [Abstract] [Full Text]  
  • Ayala-Castro, C., Saini, A., Outten, F. W. (2008). Fe-S Cluster Assembly Pathways in Bacteria. Microbiol. Mol. Biol. Rev. 72: 110-125 [Abstract] [Full Text]  
  • Browne, B. A., Ramos, A. I., Downs, D. M. (2006). PurF-Independent Phosphoribosyl Amine Formation in yjgF Mutants of Salmonella enterica Utilizes the Tryptophan Biosynthetic Enzyme Complex Anthranilate Synthase-Phosphoribosyltransferase.. J. Bacteriol. 188: 6786-6792 [Abstract] [Full Text]  
  • Schmitz, G. E., Downs, D. M. (2006). An Allele of gyrA Prevents Salmonella enterica Serovar Typhimurium from Using Succinate as a Carbon Source.. J. Bacteriol. 188: 3126-3129 [Abstract] [Full Text]  
  • Dougherty, M. J., Downs, D. M. (2004). A Mutant Allele of rpoD Results in Increased Conversion of Aminoimidazole Ribotide to Hydroxymethyl Pyrimidine in Salmonella enterica. J. Bacteriol. 186: 4034-4037 [Abstract] [Full Text]  
  • Sheehan, B. J., Bosse, J. T., Beddek, A. J., Rycroft, A. N., Kroll, J. S., Langford, P. R. (2003). Identification of Actinobacillus pleuropneumoniae Genes Important for Survival during Infection in Its Natural Host. Infect. Immun. 71: 3960-3970 [Abstract] [Full Text]  
  • Skovran, E., Downs, D. M. (2003). Lack of the ApbC or ApbE Protein Results in a Defect in Fe-S Cluster Metabolism in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 185: 98-106 [Abstract] [Full Text]  
  • Dougherty, M., Downs, D. M. (2003). The stm4066 Gene Product of Salmonella enterica Serovar Typhimurium Has Aminoimidazole Riboside (AIRs) Kinase Activity and Allows AIRs To Satisfy the Thiamine Requirement of pur Mutant Strains. J. Bacteriol. 185: 332-339 [Abstract] [Full Text]  
  • Allen, S., Zilles, J. L., Downs, D. M. (2002). Metabolic Flux in Both the Purine Mononucleotide and Histidine Biosynthetic Pathways Can Influence Synthesis of the Hydroxymethyl Pyrimidine Moiety of Thiamine in Salmonella enterica. J. Bacteriol. 184: 6130-6137 [Abstract] [Full Text]  
  • Zilles, J. L., Croal, L. R., Downs, D. M. (2000). Action of the Thiamine Antagonist Bacimethrin on Thiamine Biosynthesis. J. Bacteriol. 182: 5606-5610 [Abstract] [Full Text]  
  • Gralnick, J., Webb, E., Beck, B., Downs, D. (2000). Lesions in gshA (Encoding gamma -L-Glutamyl-L-Cysteine Synthetase) Prevent Aerobic Synthesis of Thiamine in Salmonella enterica Serovar Typhimurium LT2. J. Bacteriol. 182: 5180-5187 [Abstract] [Full Text]  
  • Skovran, E., Downs, D. M. (2000). Metabolic Defects Caused by Mutations in the isc Gene Cluster in Salmonella enterica Serovar Typhimurium: Implications for Thiamine Synthesis. J. Bacteriol. 182: 3896-3903 [Abstract] [Full Text]  
  • Claas, K., Weber, S., Downs, D. M. (2000). Lesions in the nuo Operon, Encoding NADH Dehydrogenase Complex I, Prevent PurF-Independent Thiamine Synthesis and Reduce Flux through the Oxidative Pentose Phosphate Pathway in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 182: 228-232 [Abstract] [Full Text]  
  • Frodyma, M., Rubio, A., Downs, D. M. (2000). Reduced Flux through the Purine Biosynthetic Pathway Results in an Increased Requirement for Coenzyme A in Thiamine Synthesis in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 182: 236-240 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Beck, B. J., Downs, D. M. (1998). The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium. J. Bacteriol. 180: 885-891 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»