This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Navarre, W. W.
Right arrow Articles by Schneewind, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Navarre, W. W.
Right arrow Articles by Schneewind, O.

 Previous Article  |  Next Article 

J. Bacteriol., 01 1996, 441-446, Vol 178, No. 2
Copyright © 1996, American Society for Microbiology

Cell wall sorting of lipoproteins in Staphylococcus aureus

WW Navarre, S Daefler and O Schneewind
Department of Microbiology and Immunology, UCLA School of Medicine 90024, USA.

Many surface proteins are thought to be anchored to the cell wall of gram-positive organisms via their C termini, while the N-terminal domains of these molecules are displayed on the bacterial surface. Cell wall anchoring of surface proteins in Staphylococcus aureus requires both an N-terminal leader peptide and a C-terminal cell wall sorting signal. By fusing the cell wall sorting of protein A to the C terminus of staphylococcal beta-lactamase, we demonstrate here that lipoproteins can also be anchored to the cell wall of S. aureus. The topology of cell wall-anchored beta-lactamase is reminiscent of that described for Braun's murein lipoprotein in that the N terminus of the polypeptide chain is membrane anchored whereas the C-terminal end is tethered to the bacterial cell wall.


This article has been cited by other articles:

  • Tschumi, A., Nai, C., Auchli, Y., Hunziker, P., Gehrig, P., Keller, P., Grau, T., Sander, P. (2009). Identification of Apolipoprotein N-Acyltransferase (Lnt) in Mycobacteria. J. Biol. Chem. 284: 27146-27156 [Abstract] [Full Text]  
  • Maresso, A. W., Schneewind, O. (2008). Sortase as a Target of Anti-Infective Therapy. Pharmacol. Rev. 60: 128-141 [Abstract] [Full Text]  
  • Marraffini, L. A., DeDent, A. C., Schneewind, O. (2006). Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 70: 192-221 [Abstract] [Full Text]  
  • Robichon, C., Vidal-Ingigliardi, D., Pugsley, A. P. (2005). Depletion of Apolipoprotein N-Acyltransferase Causes Mislocalization of Outer Membrane Lipoproteins in Escherichia coli. J. Biol. Chem. 280: 974-983 [Abstract] [Full Text]  
  • Tjalsma, H., Antelmann, H., Jongbloed, J. D.H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome. Microbiol. Mol. Biol. Rev. 68: 207-233 [Abstract] [Full Text]  
  • Grkovic, S., Brown, M. H., Hardie, K. M., Firth, N., Skurray, R. A. (2003). Stable low-copy-number Staphylococcus aureus shuttle vectors. Microbiology 149: 785-794 [Abstract] [Full Text]  
  • Fukuda, A., Matsuyama, S.-i., Hara, T., Nakayama, J., Nagasawa, H., Tokuda, H. (2002). Aminoacylation of the N-terminal Cysteine Is Essential for Lol-dependent Release of Lipoproteins from Membranes but Does Not Depend on Lipoprotein Sorting Signals. J. Biol. Chem. 277: 43512-43518 [Abstract] [Full Text]  
  • Perry, A. M., Ton-That, H., Mazmanian, S. K., Schneewind, O. (2002). Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. III. LIPID II IS AN IN VIVO PEPTIDOGLYCAN SUBSTRATE FOR SORTASE-CATALYZED SURFACE PROTEIN ANCHORING. J. Biol. Chem. 277: 16241-16248 [Abstract] [Full Text]  
  • Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., van Dijl, J. M. (2000). Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome. Microbiol. Mol. Biol. Rev. 64: 515-547 [Abstract] [Full Text]  
  • Ton-That, H., Mazmanian, S. K., Faull, K. F., Schneewind, O. (2000). Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. SORTASE CATALYZED IN VITRO TRANSPEPTIDATION REACTION USING LPXTG PEPTIDE AND NH2-GLY3 SUBSTRATES. J. Biol. Chem. 275: 9876-9881 [Abstract] [Full Text]  
  • Ton-That, H., Schneewind, O. (1999). Anchor Structure of Staphylococcal Surface Proteins. IV. INHIBITORS OF THE CELL WALL SORTING REACTION. J. Biol. Chem. 274: 24316-24320 [Abstract] [Full Text]  
  • Navarre, W. W., Schneewind, O. (1999). Surface Proteins of Gram-Positive Bacteria and Mechanisms of Their Targeting to the Cell Wall Envelope. Microbiol. Mol. Biol. Rev. 63: 174-229 [Abstract] [Full Text]  
  • Ton-That, H., Labischinski, H., Berger-Bachi, B., Schneewind, O. (1998). Anchor Structure of Staphylococcal Surface Proteins. III. ROLE OF THE FemA, FemB, AND FemX FACTORS IN ANCHORING SURFACE PROTEINS TO THE BACTERIAL CELL WALL. J. Biol. Chem. 273: 29143-29149 [Abstract] [Full Text]  
  • Ton-That, H., Faull, K. F., Schneewind, O. (1997). Anchor Structure of Staphylococcal Surface Proteins. A BRANCHED PEPTIDE THAT LINKS THE CARBOXYL TERMINUS OF PROTEINS TO THE CELL WALL. J. Biol. Chem. 272: 22285-22292 [Abstract] [Full Text]  
  • Mazmanian, S. K., Liu, G., Jensen, E. R., Lenoy, E., Schneewind, O. (2000). From the Cover: Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections. Proc. Natl. Acad. Sci. USA 97: 5510-5515 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»