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J. Bacteriol., Oct 1996, 6067-6069, Vol 178, No. 20
Copyright © 1996, American Society for Microbiology

Secondary structure of the outer membrane proteins OmpA of Escherichia coli and OprF of Pseudomonas aeruginosa

E Sugawara, M Steiert, S Rouhani and H Nikaido
Department of Molecular and Cell Biology, University of California, Berkeley, USA.

When purified without the use of ionic detergents, both OmpA and OprF proteins contained nearly 20% alpha-helical structures, which disappeared completely upon the addition of sodium dodecyl sulfate. This result suggests that the proteins fold in a similar manner, with an N-terminal, membrane-spanning beta-barrel domain and a C-terminal, globular, periplasmic domain.


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