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J. Bacteriol., Nov 1996, 6623-6627, Vol 178, No. 22
Copyright © 1996, American Society for Microbiology

Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2

CE French, S Nicklin and NC Bruce
Institute of Biotechnology, University of Cambridge, United Kingdom.

Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsaturated bond of 2- cyclohexen-1-one.


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