This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Al-Deib, A. A.
Right arrow Articles by Lloyd, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Al-Deib, A. A.
Right arrow Articles by Lloyd, R. G.

 Previous Article  |  Next Article 

J. Bacteriol., 12 1996, 6782-6789, Vol 178, No. 23
Copyright © 1996, American Society for Microbiology

Modulation of recombination and DNA repair by the RecG and PriA helicases of Escherichia coli K-12

AA Al-Deib, AA Mahdi and RG Lloyd
Department of Genetics, University of Nottingham, Queens Medical Centre, United Kingdom.

The RecG protein of Escherichia coli is a structure-specific DNA helicase that targets strand exchange intermediates in genetic recombination and drives their branch migration along the DNA. Strains carrying null mutations in recG show reduced recombination and DNA repair. Suppressors of this phenotype, called srgA, were located close to metB and shown to be alleles of priA. Suppression depends on the RecA, RecBCD, RecF, RuvAB, and RuvC recombination proteins. Nine srgA mutations were sequenced and shown to specify mutant PriA proteins with single amino acid substitutions located in or close to one of the conserved helicase motifs. The mutant proteins retain the ability to catalyze primosome assembly, as judged by the viability of recG srgA and srgA strains and their ability to support replication of plasmids based on the ColE1 replicon. Multicopy priA+ plasmids increase substantially the recombination- and repair-deficient phenotype of recG strains and confer similar phenotypes on recG srgA double mutants but not on ruvAB or wild-type strains. The multicopy effect is eliminated by K230R, C446G, and C477G substitutions in PriA. It is concluded that the 3'-5' DNA helicase/translocase activity of PriA inhibits recombination and that this effect is normally countered by RecG.


This article has been cited by other articles:

  • Tanaka, T., Masai, H. (2006). Stabilization of a Stalled Replication Fork by Concerted Actions of Two Helicases. J. Biol. Chem. 281: 3484-3493 [Abstract] [Full Text]  
  • Briggs, G. S., Mahdi, A. A., Wen, Q., Lloyd, R. G. (2005). DNA Binding by the Substrate Specificity (Wedge) Domain of RecG Helicase Suggests a Role in Processivity. J. Biol. Chem. 280: 13921-13927 [Abstract] [Full Text]  
  • Ivancic-Bace, I., Salaj-Smic, E., Brcic-Kostic, K. (2005). Effects of recJ, recQ, and recFOR Mutations on Recombination in Nuclease-Deficient recB recD Double Mutants of Escherichia coli. J. Bacteriol. 187: 1350-1356 [Abstract] [Full Text]  
  • Ivancic-Bace, I., Peharec, P., Moslavac, S., Skrobot, N., Salaj-Smic, E., Brcic-Kostic, K. (2003). RecFOR Function Is Required for DNA Repair and Recombination in a RecA Loading-Deficient recB Mutant of Escherichia coli. Genetics 163: 485-494 [Abstract] [Full Text]  
  • Tanaka, T., Mizukoshi, T., Taniyama, C., Kohda, D., Arai, K.-i., Masai, H. (2002). DNA Binding of PriA Protein Requires Cooperation of the N-terminal D-loop/Arrested-fork Binding and C-terminal Helicase Domains. J. Biol. Chem. 277: 38062-38071 [Abstract] [Full Text]  
  • Cox, M. M. (2001). Historical overview: Searching for replication help in all of the rec places. Proc. Natl. Acad. Sci. USA 98: 8173-8180 [Abstract] [Full Text]  
  • McGlynn, P., Lloyd, R. G. (2001). Rescue of stalled replication forks by RecG: Simultaneous translocation on the leading and lagging strand templates supports an active DNA unwinding model of fork reversal and Holliday junction formation. Proc. Natl. Acad. Sci. USA 98: 8227-8234 [Abstract] [Full Text]  
  • Kuzminov, A. (2001). DNA replication meets genetic exchange: Chromosomal damage and its repair by homologous recombination. Proc. Natl. Acad. Sci. USA 98: 8461-8468 [Abstract] [Full Text]  
  • Lombardo, M.-J., Rosenberg, S. M. (2000). radC102 of Escherichia coli Is an Allele of recG. J. Bacteriol. 182: 6287-6291 [Abstract] [Full Text]  
  • McGlynn, P., Mahdi, A. A., Lloyd, R. G. (2000). Characterisation of the catalytically active form of RecG helicase. Nucleic Acids Res 28: 2324-2332 [Abstract] [Full Text]  
  • Bull, H. J., McKenzie, G. J., Hastings, P. J., Rosenberg, S. M. (2000). Evidence That Stationary-Phase Hypermutation in the Escherichia coli Chromosome Is Promoted by Recombination. Genetics 154: 1427-1437 [Abstract] [Full Text]  
  • Cromie, G. A., Millar, C. B., Schmidt, K. H., Leach, D. R. F. (2000). Palindromes as Substrates for Multiple Pathways of Recombination in Escherichia coli. Genetics 154: 513-522 [Abstract] [Full Text]  
  • Sandler, S. J., Marians, K. J. (2000). Role of PriA in Replication Fork Reactivation in Escherichia coli. J. Bacteriol. 182: 9-13 [Full Text]  
  • Kuzminov, A. (1999). Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63: 751-813 [Abstract] [Full Text]  
  • Sharples, G. J., Ingleston, S. M., Lloyd, R. G. (1999). Holliday Junction Processing in Bacteria: Insights from the Evolutionary Conservation of RuvABC, RecG, and RusA. J. Bacteriol. 181: 5543-5550 [Full Text]  
  • Poteete, A. R., Fenton, A. C., Murphy, K. C. (1999). Roles of RuvC and RecG in Phage lambda Red-Mediated Recombination. J. Bacteriol. 181: 5402-5408 [Abstract] [Full Text]  
  • Foster, P. L., Rosche, W. A. (1999). Increased Episomal Replication Accounts for the High Rate of Adaptive Mutation in recD Mutants of Escherichia coli. Genetics 152: 15-30 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Whitby, M. C., Lloyd, R. G. (1998). Targeting Holliday Junctions by the RecG Branch Migration Protein of Escherichia coli. J. Biol. Chem. 273: 19729-19739 [Abstract] [Full Text]  
  • Friedman-Ohana, R., Cohen, A. (1998). Heteroduplex joint formation in Escherichia coli recombination is initiated by pairing of a 3'-ending strand. Proc. Natl. Acad. Sci. USA 95: 6909-6914 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»