This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Membrillo-Hernandez, J.
Right arrow Articles by Poole, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Membrillo-Hernandez, J.
Right arrow Articles by Poole, R. K.

 Previous Article  |  Next Article 

J. Bacteriol., May 1997, 3164-3170, Vol 179, No. 10
Copyright © 1997, American Society for Microbiology

Paraquat regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12 is SoxRS independent but modulated by sigma S

J Membrillo-Hernandez, SO Kim, GM Cook and RK Poole
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, United Kingdom.

We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expression, and maximal induction was observed only after 20 min of exposure to paraquat. Neither a mutation in soxS nor one in soxR prevented the paraquat- dependent increase in phi(hmp-lacZ) expression, but either mutant allele delayed full expression of phi(hmp-lacZ) activity after paraquat addition. Induction of hmp by paraquat was demonstrated in aerobically grown cultures during exponential growth and the stationary phase, thus revealing two Sox-independent regulatory mechanisms. Induction of hmp by paraquat in the stationary phase was dependent on the global regulator of stationary-phase gene expression, RpoS (sigma S). However, a mutation in rpoS did not prevent an increase in hmp expression by paraquat in exponentially growing cells. Induction of sigma S in the exponential phase by heat shock also induced phi(hmp-lacZ) expression in the presence of paraquat, supporting the role of sigma S in one of the regulatory mechanisms. Mutations in oxyR or rob, known regulators of several stress promoters in E. coli, had no effect on the induction of hmp by paraquat. Other known superoxide-generating agents (plumbagin, menadione, and phenazine methosulfate) were not effective in inducing hmp expression.


This article has been cited by other articles:

  • Elvers, K. T., Wu, G., Gilberthorpe, N. J., Poole, R. K., Park, S. F. (2004). Role of an Inducible Single-Domain Hemoglobin in Mediating Resistance to Nitric Oxide and Nitrosative Stress in Campylobacter jejuni and Campylobacter coli. J. Bacteriol. 186: 5332-5341 [Abstract] [Full Text]  
  • Ullmann, B. D., Myers, H., Chiranand, W., Lazzell, A. L., Zhao, Q., Vega, L. A., Lopez-Ribot, J. L., Gardner, P. R., Gustin, M. C. (2004). Inducible Defense Mechanism against Nitric Oxide in Candida albicans. Eukaryot Cell 3: 715-723 [Abstract] [Full Text]  
  • Frey, A. D., Farres, J., Bollinger, C. J. T., Kallio, P. T. (2002). Bacterial Hemoglobins and Flavohemoglobins for Alleviation of Nitrosative Stress in Escherichia coli. Appl. Environ. Microbiol. 68: 4835-4840 [Abstract] [Full Text]  
  • Stevanin, T. M., Poole, R. K., Demoncheaux, E. A. G., Read, R. C. (2002). Flavohemoglobin Hmp Protects Salmonella enterica Serovar Typhimurium from Nitric Oxide-Related Killing by Human Macrophages. Infect. Immun. 70: 4399-4405 [Abstract] [Full Text]  
  • Pomposiello, P. J., Bennik, M. H. J., Demple, B. (2001). Genome-Wide Transcriptional Profiling of the Escherichia coli Responses to Superoxide Stress and Sodium Salicylate. J. Bacteriol. 183: 3890-3902 [Abstract] [Full Text]  
  • Hu, Y., Butcher, P. D., Mangan, J. A., Rajandream, M.-A., Coates, A. R. M. (1999). Regulation of hmp Gene Transcription in Mycobacterium tuberculosis: Effects of Oxygen Limitation and Nitrosative and Oxidative Stress. J. Bacteriol. 181: 3486-3493 [Abstract] [Full Text]  
  • Membrillo-Hernandez, J., Coopamah, M. D., Anjum, M. F., Stevanin, T. M., Kelly, A., Hughes, M. N., Poole, R. K. (1999). The Flavohemoglobin of Escherichia coli Confers Resistance to a Nitrosating Agent, a "Nitric Oxide Releaser," and Paraquat and Is Essential for Transcriptional Responses to Oxidative Stress. J. Biol. Chem. 274: 748-754 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Crawford, M. J., Goldberg, D. E. (1998). Role for the Salmonella Flavohemoglobin in Protection from Nitric Oxide. J. Biol. Chem. 273: 12543-12547 [Abstract] [Full Text]  
  • Buisson, N., Labbe-Bois, R. (1998). Flavohemoglobin Expression and Function in Saccharomyces cerevisiae. NO RELATIONSHIP WITH RESPIRATION AND COMPLEX RESPONSE TO OXIDATIVE STRESS. J. Biol. Chem. 273: 9527-9533 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»