This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, S. J.
Right arrow Articles by Gunsalus, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, S. J.
Right arrow Articles by Gunsalus, R. P.

 Previous Article  |  Next Article 

J. Bacteriol., Jul 1997, 4138-4142, Vol 179, No. 13
Copyright © 1997, American Society for Microbiology

Aerobic regulation of the sucABCD genes of Escherichia coli, which encode alpha-ketoglutarate dehydrogenase and succinyl coenzyme A synthetase: roles of ArcA, Fnr, and the upstream sdhCDAB promoter

SJ Park, G Chao and RP Gunsalus
Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles 90095, USA.

The sucABCD genes of Escherichia coli encode subunits for two enzymes of the tricarboxylic acid (TCA) cycle, alpha-ketoglutarate dehydrogenase (sucAB) and succinyl coenzyme A synthetase (sucCD). To examine how these genes are expressed in response to changes in oxygen and carbon availability, a set of sucA-lacZ, sucC-lacZ, sdhCDAB-sucA- lacZ, and sdhC-lacZ fusions were constructed and analyzed in vivo. While the expression of a sucA-lacZ fusion was low under all cell growth conditions tested, the expression of the sucA gene from the upstream sdhC promoter was considerably higher and varied by up to 14- fold depending on the carbon substrate used. Expression of the sdhCDAB- sucA-lacZ fusion varied by fourfold in response to oxygen. In contrast, no expression was seen from a sucC-lacZ reporter fusion, indicating that no promoter immediately precedes the sucCD genes. Taken together, these findings demonstrate that the oxygen and carbon control of sucABCD gene expression occurs by transcriptional regulation of the upstream sdhC promoter. The weaker sucA promoter provides an additional low constitutive level of sucABCD gene expression to supplement transcription from the sdhC promoter. The negative control of sucABCD gene expression seen under anaerobic conditions, like that for the sdhCDAB genes, is provided by the arcA and fnr gene products. These findings establish that the differential expression of eight genes for three of the TCA cycle enzymes in E. coli is controlled from one regulatory element.


This article has been cited by other articles:

  • Mercado-Lubo, R., Leatham, M. P., Conway, T., Cohen, P. S. (2009). Salmonella enterica Serovar Typhimurium Mutants Unable To Convert Malate to Pyruvate and Oxaloacetate Are Avirulent and Immunogenic in BALB/c Mice. Infect. Immun. 77: 1397-1405 [Abstract] [Full Text]  
  • Cho, B.-K., Knight, E. M., Barrett, C. L., Palsson, B. O. (2008). Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts. Genome Res 18: 900-910 [Abstract] [Full Text]  
  • Mercado-Lubo, R., Gauger, E. J., Leatham, M. P., Conway, T., Cohen, P. S. (2008). A Salmonella enterica Serovar Typhimurium Succinate Dehydrogenase/Fumarate Reductase Double Mutant Is Avirulent and Immunogenic in BALB/c Mice. Infect. Immun. 76: 1128-1134 [Abstract] [Full Text]  
  • Liu, S., Hu, X., Lohrke, S. M., Baker, C. J., Buyer, J. S., de Souza, J. T., Roberts, D. P. (2007). Role of sdhA and pfkA and catabolism of reduced carbon during colonization of cucumber roots by Enterobacter cloacae. Microbiology 153: 3196-3209 [Abstract] [Full Text]  
  • Fink, R. C., Evans, M. R., Porwollik, S., Vazquez-Torres, A., Jones-Carson, J., Troxell, B., Libby, S. J., McClelland, M., Hassan, H. M. (2007). FNR Is a Global Regulator of Virulence and Anaerobic Metabolism in Salmonella enterica Serovar Typhimurium (ATCC 14028s). J. Bacteriol. 189: 2262-2273 [Abstract] [Full Text]  
  • Partridge, J. D., Scott, C., Tang, Y., Poole, R. K., Green, J. (2006). Escherichia coli Transcriptome Dynamics during the Transition from Anaerobic to Aerobic Conditions. J. Biol. Chem. 281: 27806-27815 [Abstract] [Full Text]  
  • Tchawa Yimga, M., Leatham, M. P., Allen, J. H., Laux, D. C., Conway, T., Cohen, P. S. (2006). Role of Gluconeogenesis and the Tricarboxylic Acid Cycle in the Virulence of Salmonella enterica Serovar Typhimurium in BALB/c Mice. Infect. Immun. 74: 1130-1140 [Abstract] [Full Text]  
  • Hommes, N. G., Kurth, E. G., Sayavedra-Soto, L. A., Arp, D. J. (2006). Disruption of sucA, Which Encodes the E1 Subunit of {alpha}-Ketoglutarate Dehydrogenase, Affects the Survival of Nitrosomonas europaea in Stationary Phase. J. Bacteriol. 188: 343-347 [Abstract] [Full Text]  
  • Perrenoud, A., Sauer, U. (2005). Impact of Global Transcriptional Regulation by ArcA, ArcB, Cra, Crp, Cya, Fnr, and Mlc on Glucose Catabolism in Escherichia coli. J. Bacteriol. 187: 3171-3179 [Abstract] [Full Text]  
  • Salmon, K. A., Hung, S.-p., Steffen, N. R., Krupp, R., Baldi, P., Hatfield, G. W., Gunsalus, R. P. (2005). Global Gene Expression Profiling in Escherichia coli K12: EFFECTS OF OXYGEN AVAILABILITY AND ArcA. J. Biol. Chem. 280: 15084-15096 [Abstract] [Full Text]  
  • Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  
  • Kang, Y., Weber, K. D., Qiu, Y., Kiley, P. J., Blattner, F. R. (2005). Genome-Wide Expression Analysis Indicates that FNR of Escherichia coli K-12 Regulates a Large Number of Genes of Unknown Function. J. Bacteriol. 187: 1135-1160 [Abstract] [Full Text]  
  • Gosset, G., Zhang, Z., Nayyar, S., Cuevas, W. A., Saier, M. H. Jr. (2004). Transcriptome Analysis of Crp-Dependent Catabolite Control of Gene Expression in Escherichia coli. J. Bacteriol. 186: 3516-3524 [Abstract] [Full Text]  
  • Causey, T. B., Zhou, S., Shanmugam, K. T., Ingram, L. O. (2003). Inaugural Article: Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production. Proc. Natl. Acad. Sci. USA 100: 825-832 [Abstract] [Full Text]  
  • Verhamme, D. T., Arents, J. C., Postma, P. W., Crielaard, W., Hellingwerf, K. J. (2002). Investigation of in vivo cross-talk between key two-component systems of Escherichia coli. Microbiology 148: 69-78 [Abstract] [Full Text]  
  • Kapatral, V., Bina, X., Chakrabarty, A. M. (2000). Succinyl Coenzyme A Synthetase of Pseudomonas aeruginosa with a Broad Specificity for Nucleoside Triphosphate (NTP) Synthesis Modulates Specificity for NTP Synthesis by the 12-Kilodalton Form of Nucleoside Diphosphate Kinase. J. Bacteriol. 182: 1333-1339 [Abstract] [Full Text]  
  • Chang, D.-E., Shin, S., Rhee, J.-S., Pan, J.-G. (1999). Acetate Metabolism in a pta Mutant of Escherichia coli W3110: Importance of Maintaining Acetyl Coenzyme A Flux for Growth and Survival. J. Bacteriol. 181: 6656-6663 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Arfin, S. M., Long, A. D., Ito, E. T., Tolleri, L., Riehle, M. M., Paegle, E. S., Hatfield, G. W. (2000). Global Gene Expression Profiling in Escherichia coli K12. THE EFFECTS OF INTEGRATION HOST FACTOR. J. Biol. Chem. 275: 29672-29684 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»