This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chao, G.
Right arrow Articles by Gunsalus, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chao, G.
Right arrow Articles by Gunsalus, R. P.

 Previous Article  |  Next Article 

J. Bacteriol., 07 1997, 4299-4304, Vol 179, No. 13
Copyright © 1997, American Society for Microbiology

Aerobic regulation of isocitrate dehydrogenase gene (icd) expression in Escherichia coli by the arcA and fnr gene products

G Chao, J Shen, CP Tseng, SJ Park and RP Gunsalus
Department of Microbiology and Molecular Genetics and Molecular Biology Institute, University of California, Los Angeles 90095, USA.

Isocitrate dehydrogenase, the icd gene product, has been studied extensively regarding the regulation of enzymatic activity and its relationship to the metabolic flux between the tricarboxylic acid cycle and the glyoxylate bypass. In this study, the transcriptional regulation of icd gene expression was monitored by using an icd-lacZ gene fusion and shown to vary over a 15-fold range in response to changes in oxygen and carbon availability. Anaerobic cell growth resulted in fivefold-lower icd-lacZ expression than during aerobic growth. This negative control is mediated by the arcA and fnr gene products. When different carbon compounds were used for cell growth, icd-lacZ expression varied threefold. The results of continuous cell culture studies indicated that this control may be due to variations in cell growth rate rather than to catabolite repression. DNase I footprinting at the icd promoter revealed a 42-bp ArcA-phosphate- protected region that overlaps the start site of icd transcription. Phosphorylation of ArcA considerably enhanced its binding to DNA, while ArcA-phosphate exhibited an apparent dissociation value of approximately 0.1 microM. Based on these studies, ArcA appears to function as a classical repressor of transcription by binding at a site overlapping the icd promoter during anaerobic cell growth conditions.


This article has been cited by other articles:

  • Cho, B.-K., Knight, E. M., Palsson, B. O. (2006). Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA.. Microbiology 152: 2207-2219 [Abstract] [Full Text]  
  • Stevenson, D. M., Weimer, P. J. (2005). Expression of 17 Genes in Clostridium thermocellum ATCC 27405 during Fermentation of Cellulose or Cellobiose in Continuous Culture. Appl. Environ. Microbiol. 71: 4672-4678 [Abstract] [Full Text]  
  • Perrenoud, A., Sauer, U. (2005). Impact of Global Transcriptional Regulation by ArcA, ArcB, Cra, Crp, Cya, Fnr, and Mlc on Glucose Catabolism in Escherichia coli. J. Bacteriol. 187: 3171-3179 [Abstract] [Full Text]  
  • Salmon, K. A., Hung, S.-p., Steffen, N. R., Krupp, R., Baldi, P., Hatfield, G. W., Gunsalus, R. P. (2005). Global Gene Expression Profiling in Escherichia coli K12: EFFECTS OF OXYGEN AVAILABILITY AND ArcA. J. Biol. Chem. 280: 15084-15096 [Abstract] [Full Text]  
  • Kang, Y., Weber, K. D., Qiu, Y., Kiley, P. J., Blattner, F. R. (2005). Genome-Wide Expression Analysis Indicates that FNR of Escherichia coli K-12 Regulates a Large Number of Genes of Unknown Function. J. Bacteriol. 187: 1135-1160 [Abstract] [Full Text]  
  • Liu, X., De Wulf, P. (2004). Probing the ArcA-P Modulon of Escherichia coli by Whole Genome Transcriptional Analysis and Sequence Recognition Profiling. J. Biol. Chem. 279: 12588-12597 [Abstract] [Full Text]  
  • Sun, J., Smets, I., Bernaerts, K., Van Impe, J., Vanderleyden, J., Marchal, K. (2001). Quantitative Analysis of Bacterial Gene Expression by Using the gusA Reporter Gene System. Appl. Environ. Microbiol. 67: 3350-3357 [Abstract] [Full Text]  
  • Prost, J.-F., Nègre, D., Oudot, C., Murakami, K., Ishihama, A., Cozzone, A. J., Cortay, J.-C. (1999). Cra-Dependent Transcriptional Activation of the icd Gene of Escherichia coli. J. Bacteriol. 181: 893-898 [Abstract] [Full Text]  
  • Nakano, M. M., Zuber, P., Sonenshein, A. L. (1998). Anaerobic Regulation of Bacillus subtilis Krebs Cycle Genes. J. Bacteriol. 180: 3304-3311 [Abstract] [Full Text]  
  • Arfin, S. M., Long, A. D., Ito, E. T., Tolleri, L., Riehle, M. M., Paegle, E. S., Hatfield, G. W. (2000). Global Gene Expression Profiling in Escherichia coli K12. THE EFFECTS OF INTEGRATION HOST FACTOR. J. Biol. Chem. 275: 29672-29684 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»