This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brazill, D. T. Articles by Martin, G. S. Search for Related Content PubMed PubMed Citation Articles by Brazill, D. T. Articles by Martin, G. S.

 Previous Article  |  Next Article 

J. Bacteriol., Jul 1997, 4415-4418, Vol 179, No. 13
Copyright © 1997, American Society for Microbiology

Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae

DT Brazill, J Thorner and GS Martin
Department of Molecular and Cell Biology, University of California at Berkeley, 94720, USA.

An interaction between the Saccharomyces cerevisiae protein kinase Mck1 and pyruvate kinase (Pyk1) was detected by using the two-hybrid method. Purified Mck1 was able to phosphorylate purified Pyk1 on Ser in vitro. Pyruvate kinase activity was elevated in mck1 delta cells. Several of the phenotypes of mck1 delta mutants are similar to those observed in cells overexpressing PYK1. Co-overexpression of MCK1 suppressed all of the phenotypes associated with PYK1 overexpression. These results indicate that Mck1 negatively regulates pyruvate kinase activity, possibly by direct phosphorylation.

This article has been cited by other articles:

• Portela, P., Howell, S., Moreno, S., Rossi, S. (2002). In Vivo and in Vitro Phosphorylation of Two Isoforms of Yeast Pyruvate Kinase by Protein Kinase A. J. Biol. Chem. 277: 30477-30487 [Abstract] [Full Text]  
• Rayner, T. F., Gray, J. V., Thorner, J. W. (2002). Direct and Novel Regulation of cAMP-dependent Protein Kinase by Mck1p, a Yeast Glycogen Synthase Kinase-3. J. Biol. Chem. 277: 16814-16822 [Abstract] [Full Text]  
• Xiao, Y., Mitchell, A. P. (2000). Shared Roles of Yeast Glycogen Synthase Kinase 3 Family Members in Nitrogen-Responsive Phosphorylation of Meiotic Regulator Ume6p. Mol. Cell. Biol. 20: 5447-5453 [Abstract] [Full Text]  
• Beeser, A. E., Cooper, T. G. (1999). The Dual-Specificity Protein Phosphatase Yvh1p Acts Upstream of the Protein Kinase Mck1p in Promoting Spore Development in Saccharomyces cerevisiae. J. Bacteriol. 181: 5219-5224 [Abstract] [Full Text]