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J. Bacteriol., Jul 1997, 4426-4428, Vol 179, No. 13
Copyright © 1997, American Society for Microbiology

Versatile insertion plasmids for targeted genome manipulations in bacteria: isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome

G Posfai, MD Koob, HA Kirkpatrick and FR Blattner
Institute of Biochemistry, Biological Research Center, Szeged, Hungary. posfaigy@everx.szbk.u-szeged.hu

A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.


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