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J. Bacteriol., Jul 1997, 4443-4445, Vol 179, No. 13
Copyright © 1997, American Society for Microbiology

A melibiose transporter and an operon containing its gene in Enterobacter cloacae

N Okazaki, XX Jue, H Miyake, M Kuroda, T Shimamoto and T Tsuchiya
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.

We detected inducible melibiose transport activity in cells of Enterobacter cloacae IID977. H+, but not Na+, was found to be the coupling cation for this transporter. We cloned and sequenced the gene encoding the melibiose transporter. A homology search of a protein sequence database revealed that this melibiose transporter has high sequence similarity with the lactose transporter (LacY) and the raffinose transporter (RafB) and has some similarity with the melibiose transporter (MelB) of Escherichia coli.


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