This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taniguchi, H.
Right arrow Articles by Yoshida, S. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taniguchi, H.
Right arrow Articles by Yoshida, S. I.

 Previous Article  |  Next Article 

J. Bacteriol., 08 1997, 4795-4801, Vol 179, No. 15
Copyright © 1997, American Society for Microbiology

Molecular analysis of kanamycin and viomycin resistance in Mycobacterium smegmatis by use of the conjugation system

H Taniguchi, B Chang, C Abe, Y Nikaido, Y Mizuguchi and SI Yoshida
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Yahatanishiku, Kitakyusyu, Japan. hatsumi@med.uoeh-u.ac.jp

We examined the molecular mechanisms of resistance to kanamycin and viomycin in Mycobacterium smegmatis. All of the M. smegmatis strains with high-level kanamycin resistance had a nucleotide substitution from A to G at position 1389 of the 16S rRNA gene (rrs). This position is equivalent to position 1408 of Escherichia coli, and mutation at this position is known to cause aminoglycoside resistance. Mutations from G to A or G to T at position 1473 of the M. smegmatis rrs gene were found in viomycin-resistant mutants which had been designated vicB mutants in our earlier studies. Using the M. smegmatis conjugation system, we confirmed that these mutations indeed contributed to kanamycin and viomycin resistance, and kanamycin susceptibility was dominant over resistance in a heterogenomic strain. Additional experiments showed that three of four Mycobacterium tuberculosis strains with high-level kanamycin resistance had a mutation from A to G at position 1400, which was equivalent to position 1389 of M. smegmatis.


This article has been cited by other articles:

  • Jugheli, L., Bzekalava, N., de Rijk, P., Fissette, K., Portaels, F., Rigouts, L. (2009). High Level of Cross-Resistance between Kanamycin, Amikacin, and Capreomycin among Mycobacterium tuberculosis Isolates from Georgia and a Close Relation with Mutations in the rrs Gene. Antimicrob. Agents Chemother. 53: 5064-5068 [Abstract] [Full Text]  
  • Zaunbrecher, M. A., Sikes, R. D. Jr., Metchock, B., Shinnick, T. M., Posey, J. E. (2009). Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin resistance in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 106: 20004-20009 [Abstract] [Full Text]  
  • Louw, G. E., Warren, R. M., Gey van Pittius, N. C., McEvoy, C. R. E., Van Helden, P. D., Victor, T. C. (2009). A Balancing Act: Efflux/Influx in Mycobacterial Drug Resistance. Antimicrob. Agents Chemother. 53: 3181-3189 [Full Text]  
  • Monshupanee, T., Gregory, S. T., Douthwaite, S., Chungjatupornchai, W., Dahlberg, A. E. (2008). Mutations in Conserved Helix 69 of 23S rRNA of Thermus thermophilus That Affect Capreomycin Resistance but Not Posttranscriptional Modifications. J. Bacteriol. 190: 7754-7761 [Abstract] [Full Text]  
  • Felnagle, E. A., Rondon, M. R., Berti, A. D., Crosby, H. A., Thomas, M. G. (2007). Identification of the Biosynthetic Gene Cluster and an Additional Gene for Resistance to the Antituberculosis Drug Capreomycin. Appl. Environ. Microbiol. 73: 4162-4170 [Abstract] [Full Text]  
  • Maus, C. E., Plikaytis, B. B., Shinnick, T. M. (2005). Molecular Analysis of Cross-Resistance to Capreomycin, Kanamycin, Amikacin, and Viomycin in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 49: 3192-3197 [Abstract] [Full Text]  
  • Gregory, S. T., Carr, J. F., Rodriguez-Correa, D., Dahlberg, A. E. (2005). Mutational Analysis of 16S and 23S rRNA Genes of Thermus thermophilus. J. Bacteriol. 187: 4804-4812 [Abstract] [Full Text]  
  • Gregory, S. T., Carr, J. F., Dahlberg, A. E. (2005). A Mutation in the Decoding Center of Thermus thermophilus 16S rRNA Suggests a Novel Mechanism of Streptomycin Resistance. J. Bacteriol. 187: 2200-2202 [Abstract] [Full Text]  
  • Maus, C. E., Plikaytis, B. B., Shinnick, T. M. (2005). Mutation of tlyA Confers Capreomycin Resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 49: 571-577 [Abstract] [Full Text]  
  • Kruuner, A., Jureen, P., Levina, K., Ghebremichael, S., Hoffner, S. (2003). Discordant Resistance to Kanamycin and Amikacin in Drug-Resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 47: 2971-2973 [Abstract] [Full Text]  
  • Recht, M. I., Puglisi, J. D. (2001). Aminoglycoside Resistance with Homogeneous and Heterogeneous Populations of Antibiotic-Resistant Ribosomes. Antimicrob. Agents Chemother. 45: 2414-2419 [Abstract] [Full Text]  
  • Hirano, K., Abe, C., Takahashi, M. (1999). Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis Strains Isolated Mostly in Asian Countries and Their Rapid Detection by Line Probe Assay. J. Clin. Microbiol. 37: 2663-2666 [Abstract] [Full Text]  
  • Prammananan, T., Sander, P., Springer, B., Böttger, E. C. (1999). RecA-Mediated Gene Conversion and Aminoglycoside Resistance in Strains Heterozygous for rRNA. Antimicrob. Agents Chemother. 43: 447-453 [Abstract] [Full Text]  
  • Suzuki, Y., Katsukawa, C., Tamaru, A., Abe, C., Makino, M., Mizuguchi, Y., Taniguchi, H. (1998). Detection of Kanamycin-Resistant Mycobacterium tuberculosis by Identifying Mutations in the 16S rRNA Gene. J. Clin. Microbiol. 36: 1220-1225 [Abstract] [Full Text]  
  • Gustafsson, C., Persson, B. C. (1998). Identification of the rrmA Gene Encoding the 23S rRNA m1G745 Methyltransferase in Escherichia coli and Characterization of an m1G745-Deficient Mutant. J. Bacteriol. 180: 359-365 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»