This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fukui, T.
Right arrow Articles by Doi, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukui, T.
Right arrow Articles by Doi, Y.

 Previous Article  |  Next Article 

J. Bacteriol., 08 1997, 4821-4830, Vol 179, No. 15
Copyright © 1997, American Society for Microbiology

Cloning and analysis of the poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) biosynthesis genes of Aeromonas caviae

T Fukui and Y Doi
Polymer Chemistry Laboratory, Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama, Japan.

A 5.0-kbp EcoRV-EcoRI restriction fragment was cloned and analyzed from genomic DNA of Aeromonas caviae, a bacterium producing a copolyester of (R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyhexanoate (3HHx) [P(3HB-co- 3HHx)] from alkanoic acids or oils. The nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (PHA) synthase gene (phaC(Ac) [i.e., the phaC gene from A. caviae]) together with four open reading frames (ORF1, -3, -4, and -5) and one putative promoter region. The cloned fragments could not only complement PHA-negative mutants of Alcaligenes eutrophus and Pseudomonas putida, but also confer the ability to synthesize P(3HB-co-3HHx) from octanoate or hexanoate on the mutants' hosts. Furthermore, coexpression of ORF1 and ORF3 genes with phaC(Ac) in the A. eutrophus mutant resulted in a decrease in the polyester content of the cells. Escherichia coli expressing ORF3 showed (R)-enoyl-coenzyme A (CoA) hydratase activity, suggesting that (R)-3- hydroxyacyl-CoA monomer units are supplied via the (R)-specific hydration of enoyl-CoA in A. caviae. The transconjugant of the A. eutrophus mutant expressing only phaC(Ac) effectively accumulated P(3HB- co-3HHx) up to 96 wt% of the cellular dry weight from octanoate in one- step cultivation.


This article has been cited by other articles:

  • Zarzycki, J., Schlichting, A., Strychalsky, N., Muller, M., Alber, B. E., Fuchs, G. (2008). Mesaconyl-Coenzyme A Hydratase, a New Enzyme of Two Central Carbon Metabolic Pathways in Bacteria. J. Bacteriol. 190: 1366-1374 [Abstract] [Full Text]  
  • Autio, K. J., Kastaniotis, A. J., Pospiech, H., Miinalainen, I. J., Schonauer, M. S., Dieckmann, C. L., Hiltunen, J. K. (2008). An ancient genetic link between vertebrate mitochondrial fatty acid synthesis and RNA processing. FASEB J. 22: 569-578 [Abstract] [Full Text]  
  • Brown, A. K., Papaemmanouil, A., Bhowruth, V., Bhatt, A., Dover, L. G., Besra, G. S. (2007). Flavonoid inhibitors as novel antimycobacterial agents targeting Rv0636, a putative dehydratase enzyme involved in Mycobacterium tuberculosis fatty acid synthase II. Microbiology 153: 3314-3322 [Abstract] [Full Text]  
  • Arai, Y., Shikanai, T., Doi, Y., Yoshida, S., Yamaguchi, I., Nakashita, H. (2004). Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid. Plant Cell Physiol 45: 1176-1184 [Abstract] [Full Text]  
  • Sheu, D.-S., Lee, C.-Y. (2004). Altering the Substrate Specificity of Polyhydroxyalkanoate Synthase 1 Derived from Pseudomonas putida GPo1 by Localized Semirandom Mutagenesis. J. Bacteriol. 186: 4177-4184 [Abstract] [Full Text]  
  • Koski, M. K., Haapalainen, A. M., Hiltunen, J. K., Glumoff, T. (2004). A Two-domain Structure of One Subunit Explains Unique Features of Eukaryotic Hydratase 2. J. Biol. Chem. 279: 24666-24672 [Abstract] [Full Text]  
  • Nomura, C. T., Taguchi, K., Taguchi, S., Doi, Y. (2004). Coexpression of Genetically Engineered 3-Ketoacyl-ACP Synthase III (fabH) and Polyhydroxyalkanoate Synthase (phaC) Genes Leads to Short-Chain-Length-Medium-Chain-Length Polyhydroxyalkanoate Copolymer Production from Glucose in Escherichia coli JM109. Appl. Environ. Microbiol. 70: 999-1007 [Abstract] [Full Text]  
  • Tsuge, T., Hisano, T., Taguchi, S., Doi, Y. (2003). Alteration of Chain Length Substrate Specificity of Aeromonas caviae R-Enantiomer-Specific Enoyl-Coenzyme A Hydratase through Site-Directed Mutagenesis. Appl. Environ. Microbiol. 69: 4830-4836 [Abstract] [Full Text]  
  • Hisano, T., Tsuge, T., Fukui, T., Iwata, T., Miki, K., Doi, Y. (2003). Crystal Structure of the (R)-Specific Enoyl-CoA Hydratase from Aeromonas caviae Involved in Polyhydroxyalkanoate Biosynthesis. J. Biol. Chem. 278: 617-624 [Abstract] [Full Text]  
  • Takase, K., Taguchi, S., Doi, Y. (2003). Enhanced Synthesis of Poly(3-hydroxybutyrate) in Recombinant Escherichia coli by Means of Error-Prone PCR Mutagenesis, Saturation Mutagenesis, and In Vitro Recombination of the Type II Polyhydroxyalkanoate Synthase Gene. J Biochem 133: 139-145 [Abstract] [Full Text]  
  • Arai, Y., Nakashita, H., Suzuki, Y., Kobayashi, Y., Shimizu, T., Yasuda, M., Doi, Y., Yamaguchi, I. (2002). Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-Chain-Length Intermediates of {beta}-Oxidation. Plant Cell Physiol 43: 555-562 [Abstract] [Full Text]  
  • Kichise, T., Taguchi, S., Doi, Y. (2002). Enhanced Accumulation and Changed Monomer Composition in Polyhydroxyalkanoate (PHA) Copolyester by In Vitro Evolution of Aeromonas caviae PHA Synthase. Appl. Environ. Microbiol. 68: 2411-2419 [Abstract] [Full Text]  
  • Ewering, C., Lutke-Eversloh, T., Luftmann, H., Steinbuchel, A. (2002). Identification of novel sulfur-containing bacterial polyesters: biosynthesis of poly(3-hydroxy-S-propyl-{omega}-thioalkanoates) containing thioether linkages in the side chains. Microbiology 148: 1397-1406 [Abstract] [Full Text]  
  • McCool, G. J., Cannon, M. C. (2001). PhaC and PhaR Are Required for Polyhydroxyalkanoic Acid Synthase Activity in Bacillus megaterium. J. Bacteriol. 183: 4235-4243 [Abstract] [Full Text]  
  • Taroncher-Oldenburg, G., Nishina, K., Stephanopoulos, G. (2000). Identification and Analysis of the Polyhydroxyalkanoate-Specific beta -Ketothiolase and Acetoacetyl Coenzyme A Reductase Genes in the Cyanobacterium Synechocystis sp. Strain PCC6803. Appl. Environ. Microbiol. 66: 4440-4448 [Abstract] [Full Text]  
  • Sheu, D.-S., Wang, Y.-T., Lee, C.-Y. (2000). Rapid detection of polyhydroxyalkanoate-accumulating bacteria isolated from the environment by colony PCR. Microbiology 146: 2019-2025 [Abstract] [Full Text]  
  • Fiedler, S., Steinbüchel, A., Rehm, B. H. A. (2000). PhaG-Mediated Synthesis of Poly(3-Hydroxyalkanoates) Consisting of Medium-Chain-Length Constituents from Nonrelated Carbon Sources in Recombinant Pseudomonas fragi. Appl. Environ. Microbiol. 66: 2117-2124 [Abstract] [Full Text]  
  • Ren, Q., Sierro, N., Kellerhals, M., Kessler, B., Witholt, B. (2000). Properties of Engineered Poly-3-Hydroxyalkanoates Produced in Recombinant Escherichia coli Strains. Appl. Environ. Microbiol. 66: 1311-1320 [Abstract] [Full Text]  
  • Garcia, B., Olivera, E. R., Minambres, B., Fernandez-Valverde, M., Canedo, L. M., Prieto, M. A., Garcia, J. L., Martinez, M., Luengo, J. M. (1999). Novel Biodegradable Aromatic Plastics from a Bacterial Source. GENETIC AND BIOCHEMICAL STUDIES ON A ROUTE OF THE PHENYLACETYL-CoA CATABOLON. J. Biol. Chem. 274: 29228-29241 [Abstract] [Full Text]  
  • Kolibachuk, D., Miller, A., Dennis, D. (1999). Cloning, Molecular Analysis, and Expression of the Polyhydroxyalkanoic Acid Synthase (phaC) Gene from Chromobacterium violaceum. Appl. Environ. Microbiol. 65: 3561-3565 [Abstract] [Full Text]  
  • Maehara, A., Ueda, S., Nakano, H., Yamane, T. (1999). Analyses of a Polyhydroxyalkanoic Acid Granule-Associated 16-Kilodalton Protein and Its Putative Regulator in the pha Locus of Paracoccus denitrificans. J. Bacteriol. 181: 2914-2921 [Abstract] [Full Text]  
  • Madison, L. L., Huisman, G. W. (1999). Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic. Microbiol. Mol. Biol. Rev. 63: 21-53 [Abstract] [Full Text]  
  • Matsusaki, H., Manji, S., Taguchi, K., Kato, M., Fukui, T., Doi, Y. (1998). Cloning and Molecular Analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) Biosynthesis Genes in Pseudomonas sp. Strain 61-3. J. Bacteriol. 180: 6459-6467 [Abstract] [Full Text]  
  • Choi, J.-i., Lee, S. Y., Han, K. (1998). Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli. Appl. Environ. Microbiol. 64: 4897-4903 [Abstract] [Full Text]  
  • Sudesh, K., Fukui, T., Doi, Y. (1998). Genetic Analysis of Comamonas acidovorans Polyhydroxyalkanoate Synthase and Factors Affecting the Incorporation of 4-Hydroxybutyrate Monomer. Appl. Environ. Microbiol. 64: 3437-3443 [Abstract] [Full Text]  
  • Fukui, T., Shiomi, N., Doi, Y. (1998). Expression and Characterization of (R)-Specific Enoyl Coenzyme A Hydratase Involved in Polyhydroxyalkanoate Biosynthesis by Aeromonas caviae. J. Bacteriol. 180: 667-673 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»