This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, S. M.
Right arrow Articles by Abdelal, A. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, S. M.
Right arrow Articles by Abdelal, A. T.

 Previous Article  |  Next Article 

J. Bacteriol., 09 1997, 5300-5308, Vol 179, No. 17
Copyright © 1997, American Society for Microbiology

Cloning and characterization of argR, a gene that participates in regulation of arginine biosynthesis and catabolism in Pseudomonas aeruginosa PAO1

SM Park, CD Lu and AT Abdelal
Department of Biology, Georgia State University, Atlanta 30303, USA.

Gel retardation experiments indicated the presence in Pseudomonas aeruginosa cell extracts of an arginine-inducible DNA-binding protein that interacts with the control regions for the car and argF operons, encoding carbamoylphosphate synthetase and anabolic ornithine carbamoyltransferase, respectively. Both enzymes are required for arginine biosynthesis. The use of a combination of transposon mutagenesis and arginine hydroxamate selection led to the isolation of a regulatory mutant that was impaired in the formation of the DNA- binding protein and in which the expression of an argF::lacZ fusion was not controlled by arginine. Experiments with various subclones led to the conclusion that the insertion affected the expression of an arginine regulatory gene, argR, that encodes a polypeptide with significant homology to the AraC/XylS family of regulatory proteins. Determination of the nucleotide sequence of the flanking regions showed that argR is the sixth and terminal gene of an operon for transport of arginine. The argR gene was inactivated by gene replacement, using a gentamicin cassette. Inactivation of argR abolished arginine control of the biosynthetic enzymes encoded by the car and argF operons. Furthermore, argR inactivation abolished the induction of several enzymes of the arginine succinyltransferase pathway, which is considered the major route for arginine catabolism under aerobic conditions. Consistent with this finding and unlike the parent strain, the argR::Gm derivative was unable to utilize arginine or ornithine as the sole carbon source. The combined data indicate a major role for ArgR in the control of arginine biosynthesis and aerobic catabolism.


This article has been cited by other articles:

  • Barrow, K., Kwon, D. H. (2009). Alterations in Two-Component Regulatory Systems of phoPQ and pmrAB Are Associated with Polymyxin B Resistance in Clinical Isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53: 5150-5154 [Abstract] [Full Text]  
  • Li, C., Wally, H., Miller, S. J., Lu, C.-D. (2009). The Multifaceted Proteins MvaT and MvaU, Members of the H-NS Family, Control Arginine Metabolism, Pyocyanin Synthesis, and Prophage Activation in Pseudomonas aeruginosa PAO1. J. Bacteriol. 191: 6211-6218 [Abstract] [Full Text]  
  • Wargo, M. J., Hogan, D. A. (2009). Identification of genes required for Pseudomonas aeruginosa carnitine catabolism. Microbiology 155: 2411-2419 [Abstract] [Full Text]  
  • Behrends, V., Ebbels, T. M. D., Williams, H. D., Bundy, J. G. (2009). Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization. Appl. Environ. Microbiol. 75: 2453-2463 [Abstract] [Full Text]  
  • Chou, H. T., Kwon, D.-H., Hegazy, M., Lu, C.-D. (2008). Transcriptome Analysis of Agmatine and Putrescine Catabolism in Pseudomonas aeruginosa PAO1. J. Bacteriol. 190: 1966-1975 [Abstract] [Full Text]  
  • Li, W., Lu, C.-D. (2007). Regulation of Carbon and Nitrogen Utilization by CbrAB and NtrBC Two-Component Systems in Pseudomonas aeruginosa. J. Bacteriol. 189: 5413-5420 [Abstract] [Full Text]  
  • Yang, Z., Lu, C.-D. (2007). Functional Genomics Enables Identification of Genes of the Arginine Transaminase Pathway in Pseudomonas aeruginosa. J. Bacteriol. 189: 3945-3953 [Abstract] [Full Text]  
  • Elbahloul, Y., Steinbuchel, A. (2006). Engineering the Genotype of Acinetobacter sp. Strain ADP1 To Enhance Biosynthesis of Cyanophycin. Appl. Environ. Microbiol. 72: 1410-1419 [Abstract] [Full Text]  
  • Hashim, S., Kwon, D.-H., Abdelal, A., Lu, C.-D. (2004). The Arginine Regulatory Protein Mediates Repression by Arginine of the Operons Encoding Glutamate Synthase and Anabolic Glutamate Dehydrogenase in Pseudomonas aeruginosa. J. Bacteriol. 186: 3848-3854 [Abstract] [Full Text]  
  • Lu, C.-D., Yang, Z., Li, W. (2004). Transcriptome Analysis of the ArgR Regulon in Pseudomonas aeruginosa. J. Bacteriol. 186: 3855-3861 [Abstract] [Full Text]  
  • Hernandez-Flores, J. L., Lopez-Lopez, K., Garciduenas-Pina, R., Jofre-Garfias, A. E., Alvarez-Morales, A. (2004). The Global Arginine Regulator ArgR Controls Expression of argF in Pseudomonas syringae pv. phaseolicola but Is Not Required for the Synthesis of Phaseolotoxin or for the Regulated Expression of argK. J. Bacteriol. 186: 3653-3655 [Abstract] [Full Text]  
  • Lopez-Lopez, K., Hernandez-Flores, J. L., Cruz-Aguilar, M., Alvarez-Morales, A. (2004). In Pseudomonas syringae pv. phaseolicola, Expression of the argK Gene, Encoding the Phaseolotoxin-Resistant Ornithine Carbamoyltransferase, Is Regulated Indirectly by Temperature and Directly by a Precursor Resembling Carbamoylphosphate. J. Bacteriol. 186: 146-153 [Abstract] [Full Text]  
  • Barcelona-Andres, B., Marina, A., Rubio, V. (2002). Gene Structure, Organization, Expression, and Potential Regulatory Mechanisms of Arginine Catabolism in Enterococcus faecalis. J. Bacteriol. 184: 6289-6300 [Abstract] [Full Text]  
  • McCue, L. A., Thompson, W., Carmack, C. S., Lawrence, C. E. (2002). Factors Influencing the Identification of Transcription Factor Binding Sites by Cross-Species Comparison. Genome Res 12: 1523-1532 [Abstract] [Full Text]  
  • Lu, C.-D., Itoh, Y., Nakada, Y., Jiang, Y. (2002). Functional Analysis and Regulation of the Divergent spuABCDEFGH-spuI Operons for Polyamine Uptake and Utilization in Pseudomonas aeruginosa PAO1. J. Bacteriol. 184: 3765-3773 [Abstract] [Full Text]  
  • Kiupakis, A. K., Reitzer, L. (2002). ArgR-Independent Induction and ArgR-Dependent Superinduction of the astCADBE Operon in Escherichia coli. J. Bacteriol. 184: 2940-2950 [Abstract] [Full Text]  
  • Nakada, Y., Jiang, Y., Nishijyo, T., Itoh, Y., Lu, C.-D. (2001). Molecular Characterization and Regulation of the aguBA Operon, Responsible for Agmatine Utilization in Pseudomonas aeruginosa PAO1. J. Bacteriol. 183: 6517-6524 [Abstract] [Full Text]  
  • Lu, C.-D., Abdelal, A. T. (2001). The gdhB Gene of Pseudomonas aeruginosa Encodes an Arginine-Inducible NAD+-Dependent Glutamate Dehydrogenase Which Is Subject to Allosteric Regulation. J. Bacteriol. 183: 490-499 [Abstract] [Full Text]  
  • Cowles, C. E., Nichols, N. N., Harwood, C. S. (2000). BenR, a XylS Homologue, Regulates Three Different Pathways of Aromatic Acid Degradation in Pseudomonas putida. J. Bacteriol. 182: 6339-6346 [Abstract] [Full Text]  
  • Xu, Y., Liang, Z., Legrain, C., Rüger, H. J., Glansdorff, N. (2000). Evolution of Arginine Biosynthesis in the Bacterial Domain: Novel Gene-Enzyme Relationships from Psychrophilic Moritella Strains (Vibrionaceae) and Evolutionary Significance of N-alpha -Acetyl Ornithinase. J. Bacteriol. 182: 1609-1615 [Abstract] [Full Text]  
  • Ochs, M. M., Lu, C.-D., Hancock, R. E. W., Abdelal, A. T. (1999). Amino Acid-Mediated Induction of the Basic Amino Acid-Specific Outer Membrane Porin OprD from Pseudomonas aeruginosa. J. Bacteriol. 181: 5426-5432 [Abstract] [Full Text]  
  • Belitsky, B. R., Sonenshein, A. L. (1999). An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis. Proc. Natl. Acad. Sci. USA 96: 10290-10295 [Abstract] [Full Text]  
  • Lu, C.-D., Winteler, H., Abdelal, A., Haas, D. (1999). The ArgR Regulatory Protein, a Helper to the Anaerobic Regulator ANR during Transcriptional Activation of the arcD Promoter in Pseudomonas aeruginosa. J. Bacteriol. 181: 2459-2464 [Abstract] [Full Text]  
  • Seoh, H. K., Tai, P. C. (1999). Catabolic Repression of secB Expression Is Positively Controlled by Cyclic AMP (cAMP) Receptor Protein-cAMP Complexes at the Transcriptional Level. J. Bacteriol. 181: 1892-1899 [Abstract] [Full Text]  
  • Lu, C.-D., Abdelal, A. T. (1999). Role of ArgR in Activation of the ast Operon, Encoding Enzymes of the Arginine Succinyltransferase Pathway in Salmonella typhimurium. J. Bacteriol. 181: 1934-1938 [Abstract] [Full Text]  
  • Nishijyo, T., Park, S.-M., Lu, C.-D., Itoh, Y., Abdelal, A. T. (1998). Molecular Characterization and Regulation of an Operon Encoding a System for Transport of Arginine and Ornithine and the ArgR Regulatory Protein in Pseudomonas aeruginosa. J. Bacteriol. 180: 5559-5566 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»