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J. Bacteriol., Sep 1997, 5639-5642, Vol 179, No. 17
Copyright © 1997, American Society for Microbiology

High-frequency transposition of IS1373, the insertion sequence delimiting the amplifiable element AUD2 of Streptomyces lividans

JN Volff and J Altenbuchner
Institut fur Industrielle Genetik, Universitat Stuttgart, Germany. volff@biozentrum.uni-wverzburg.de

IS1373 is the putative insertion sequence delimiting the amplifiable unit AUD2 of Streptomyces lividans. Two IS1373-derived thiostrepton- resistant transposons, Tn5492 and Tn5494, transposed into multiple sites of the S. lividans chromosome at frequencies as high as 0.4 and 1%, respectively. Hence, IS1373 is a functional insertion sequence and its unique open reading frame, insA, encodes the transposase.


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