This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muffler, A.
Right arrow Articles by Hengge-Aronis, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muffler, A.
Right arrow Articles by Hengge-Aronis, R.

 Previous Article  |  Next Article 

J. Bacteriol., 01 1997, 445-452, Vol 179, No. 2
Copyright © 1997, American Society for Microbiology

Heat shock regulation of sigmaS turnover: a role for DnaK and relationship between stress responses mediated by sigmaS and sigma32 in Escherichia coli

A Muffler, M Barth, C Marschall and R Hengge-Aronis
Department of Biology, University of Konstanz, Germany.

The cellular level of the rpoS-encoded sigmaS subunit of RNA polymerase increases in response to various stress situations that include starvation, high osmolarity, and shift to acid pH, and these different stress signals differentially affect rpoS translation and/or sigmaS stability. Here we demonstrate that sigmaS is also induced by heat shock and that this induction is exclusively due to an interference with sigmaS turnover. Some sigmaS-dependent genes exhibit similar heat shock induction, whereas others are not induced probably because they need additional regulatory factors that might not be present under conditions of heat shock or exponential growth. Despite its induction, sigmaS does not seem to contribute to heat adaptation but may induce cross-protection against different stresses. While sigmaS is not involved in the regulation of the heat shock sigma factor sigma32, the heat shock protein DnaK has a positive role in the posttranscriptional control of sigmaS. The present evidence suggests that DnaK is involved in the transduction of two of the signals that result in reduced sigmaS turnover, i.e., heat shock and carbon starvation. Heat shock induction of sigmaS also clearly indicates that a cessation of growth or even a reduction of the growth rate is not a prerequisite for the induction of sigmaS and sigmaS-dependent genes and underscores the importance of sigmaS as a general stress sigma factor.


This article has been cited by other articles:

  • White-Ziegler, C. A., Um, S., Perez, N. M., Berns, A. L., Malhowski, A. J., Young, S. (2008). Low temperature (23 {degrees}C) increases expression of biofilm-, cold-shock- and RpoS-dependent genes in Escherichia coli K-12. Microbiology 154: 148-166 [Abstract] [Full Text]  
  • Han, M.-J., Lee, S. Y. (2006). The Escherichia coli Proteome: Past, Present, and Future Prospects. Microbiol. Mol. Biol. Rev. 70: 362-439 [Abstract] [Full Text]  
  • King, T., Seeto, S., Ferenci, T. (2006). Genotype-by-Environment Interactions Influencing the Emergence of rpoS Mutations in Escherichia coli Populations. Genetics 172: 2071-2079 [Abstract] [Full Text]  
  • Imamura, S., Tanaka, K., Shirai, M., Asayama, M. (2006). Growth Phase-dependent Activation of Nitrogen-related Genes by a Control Network of Group 1 and Group 2 {sigma} Factors in a Cyanobacterium. J. Biol. Chem. 281: 2668-2675 [Abstract] [Full Text]  
  • Dominguez-Cuevas, P., Marin, P., Ramos, J. L., Marques, S. (2005). RNA Polymerase Holoenzymes Can Share a Single Transcription Start Site for the Pm Promoter: CRITICAL NUCLEOTIDES IN THE -7 TO-18 REGION ARE NEEDED TO SELECT BETWEEN RNA POLYMERASE WITH {sigma}38 OR {sigma}32. J. Biol. Chem. 280: 41315-41323 [Abstract] [Full Text]  
  • Dubern, J.-F., Lagendijk, E. L., Lugtenberg, B. J. J., Bloemberg, G. V. (2005). The Heat Shock Genes dnaK, dnaJ, and grpE Are Involved in Regulation of Putisolvin Biosynthesis in Pseudomonas putida PCL1445. J. Bacteriol. 187: 5967-5976 [Abstract] [Full Text]  
  • Zhao, K., Liu, M., Burgess, R. R. (2005). The Global Transcriptional Response of Escherichia coli to Induced {sigma}32 Protein Involves {sigma}32 Regulon Activation Followed by Inactivation and Degradation of {sigma}32 in Vivo. J. Biol. Chem. 280: 17758-17768 [Abstract] [Full Text]  
  • Imamura, S., Asayama, M., Shirai, M. (2004). In vitro transcription analysis by reconstituted cyanobacterial RNA polymerase: roles of group 1 and 2 sigma factors and a core subunit, RpoC2. GENES CELLS 9: 1175-1187 [Abstract] [Full Text]  
  • Kabir, Md. S., Sagara, T., Oshima, T., Kawagoe, Y., Mori, H., Tsunedomi, R., Yamada, M. (2004). Effects of mutations in the rpoS gene on cell viability and global gene expression under nitrogen starvation in Escherichia coli. Microbiology 150: 2543-2553 [Abstract] [Full Text]  
  • Ihssen, J., Egli, T. (2004). Specific growth rate and not cell density controls the general stress response in Escherichia coli. Microbiology 150: 1637-1648 [Abstract] [Full Text]  
  • Hengge-Aronis, R. (2002). Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase. Microbiol. Mol. Biol. Rev. 66: 373-395 [Abstract] [Full Text]  
  • Kenyon, W. J., Sayers, D. G., Humphreys, S., Roberts, M., Spector, M. P. (2002). The starvation-stress response of Salmonella enterica serovar Typhimurium requires {sigma}E-, but not CpxR-regulated extracytoplasmic functions. Microbiology 148: 113-122 [Abstract] [Full Text]  
  • Cánovas, D., Fletcher, S. A., Hayashi, M., Csonka, L. N. (2001). Role of Trehalose in Growth at High Temperature of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 183: 3365-3371 [Abstract] [Full Text]  
  • Winzer, K., Falconer, C., Garber, N. C., Diggle, S. P., Camara, M., Williams, P. (2000). The Pseudomonas aeruginosa Lectins PA-IL and PA-IIL Are Controlled by Quorum Sensing and by RpoS. J. Bacteriol. 182: 6401-6411 [Abstract] [Full Text]  
  • Srivastava, R., Cha, H. J., Peterson, M. S., Bentley, W. E. (2000). Antisense Downregulation of sigma 32 as a Transient Metabolic Controller in Escherichia coli: Effects on Yield of Active Organophosphorus Hydrolase. Appl. Environ. Microbiol. 66: 4366-4371 [Abstract] [Full Text]  
  • Vohradsky, J., Li, X.-M., Dale, G., Folcher, M., Nguyen, L., Viollier, P. H., Thompson, C. J. (2000). Developmental Control of Stress Stimulons in Streptomyces coelicolor Revealed by Statistical Analyses of Global Gene Expression Patterns. J. Bacteriol. 182: 4979-4986 [Abstract] [Full Text]  
  • Becker, G., Klauck, E., Hengge-Aronis, R. (1999). Regulation of RpoS proteolysis in Escherichia coli: The response regulator RssB is a recognition factor that interacts with the turnover element in RpoS. Proc. Natl. Acad. Sci. USA 96: 6439-6444 [Abstract] [Full Text]  
  • Cha, H. J., Srivastava, R., Vakharia, V. N., Rao, G., Bentley, W. E. (1999). Green Fluorescent Protein as a Noninvasive Stress Probe in Resting Escherichia coli Cells. Appl. Environ. Microbiol. 65: 409-414 [Abstract] [Full Text]  
  • Fischer, D., Teich, A., Neubauer, P., Hengge-Aronis, R. (1998). The General Stress Sigma Factor sigma S of Escherichia coli Is Induced during Diauxic Shift from Glucose to Lactose. J. Bacteriol. 180: 6203-6206 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Völker, U., Andersen, K. K., Antelmann, H., Devine, K. M., Hecker, M. (1998). One of Two OsmC Homologs in Bacillus subtilis Is Part of the sigma B-Dependent General Stress Regulon. J. Bacteriol. 180: 4212-4218 [Abstract] [Full Text]  
  • Zhou, Y., Gottesman, S. (1998). Regulation of Proteolysis of the Stationary-Phase Sigma Factor RpoS. J. Bacteriol. 180: 1154-1158 [Abstract] [Full Text]  
  • Rockabrand, D., Livers, K., Austin, T., Kaiser, R., Jensen, D., Burgess, R., Blum, P. (1998). Roles of DnaK and RpoS in Starvation-Induced Thermotolerance of Escherichia coli. J. Bacteriol. 180: 846-854 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»