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J. Bacteriol., 11 1997, 6858-6861, Vol 179, No. 21
Copyright © 1997, American Society for Microbiology

Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli

W Ebel, GJ Vaughn, HK Peters 3rd and JE Trempy
Department of Microbiology, Oregon State University, Corvallis 97331- 3804, USA.

Capsule gene (cps) expression, which normally occurs at low levels in Escherichia coli lon+ cells, increased 38-fold in lon+ cells carrying a Tn10::delta kan insertion mapping to 24 min on the E. coli chromosome. Null mutations in rcsA, rcsB, or rcsC abolished the effect of the Tn10::delta kan insertion. Sequencing of both sides of the Tn10::delta kan insertion localized the insertion to the previously reported mdoH gene, which encodes a protein involved in biosynthesis of membrane- derived oligosaccharides (MDOs). A model suggesting that the periplasmic levels of MDOs act to signal RcsC to activate cps expression is proposed.


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