This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Khudyakov, I. Articles by Wolk, C. P. Search for Related Content PubMed PubMed Citation Articles by Khudyakov, I. Articles by Wolk, C. P.

 Previous Article  |  Next Article 

J. Bacteriol., 11 1997, 6971-6978, Vol 179, No. 22
Copyright © 1997, American Society for Microbiology

hetC, a gene coding for a protein similar to bacterial ABC protein exporters, is involved in early regulation of heterocyst differentiation in Anabaena sp. strain PCC 7120

I Khudyakov and CP Wolk
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824, USA.

Transposon-generated mutant C3 of Anabaena sp. strain PCC 7120 is unable to form heterocysts upon deprivation of combined nitrogen but forms a pattern of spaced, weakly fluorescent cells after 2 days of deprivation. Sequence analysis of chromosomal DNA adjacent to the ends of transposon Tn5-1058 in mutant C3 showed a 1,044-amino-acid open reading frame, designated hetC, whose predicted protein product throughout its C-terminal two-thirds has extensive similarity to the HlyB family of bacterial protein exporters. Its N-terminal third is unique and does not resemble any known protein. hetC lies 1,165 bp 5' from the previously described gene hetP. Reconstruction of the C3 mutation and its complementation in trans with a wild-type copy of hetC confirmed that hetC has an essential regulatory role early in heterocyst development. hetC is induced ca. 4 h after nitrogen stepdown, hours after induction of hetR. Expression of hetC depends on HetR and may depend on HetC. Highly similar sequences are present 5' from the initiation codons and in the 3' untranslated regions of hetC and of two heterocyst-specific genes, devA and hetP.

This article has been cited by other articles:

• Muro-Pastor, A. M., Flores, E., Herrero, A. (2009). NtcA-Regulated Heterocyst Differentiation Genes hetC and devB from Anabaena sp. Strain PCC 7120 Exhibit a Similar Tandem Promoter Arrangement. J. Bacteriol. 191: 5765-5774 [Abstract] [Full Text]  
• Paz-Yepes, J., Flores, E., Herrero, A. (2009). Expression and Mutational Analysis of the glnB Genomic Region in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 191: 2353-2361 [Abstract] [Full Text]  
• Valladares, A., Flores, E., Herrero, A. (2008). Transcription Activation by NtcA and 2-Oxoglutarate of Three Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 190: 6126-6133 [Abstract] [Full Text]  
• Fan, Q., Lechno-Yossef, S., Ehira, S., Kaneko, T., Ohmori, M., Sato, N., Tabata, S., Wolk, C. P. (2006). Signal Transduction Genes Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120.. J. Bacteriol. 188: 6688-6693 [Abstract] [Full Text]  
• Orozco, C. C., Risser, D. D., Callahan, S. M. (2006). Epistasis Analysis of Four Genes from Anabaena sp. Strain PCC 7120 Suggests a Connection between PatA and PatS in Heterocyst Pattern Formation.. J. Bacteriol. 188: 1808-1816 [Abstract] [Full Text]  
• Wang, Y., Xu, X. (2005). Regulation by hetC of Genes Required for Heterocyst Differentiation and Cell Division in Anabaena sp. Strain PCC 7120. J. Bacteriol. 187: 8489-8493 [Abstract] [Full Text]  
• Igarashi, Y., Aoki, K. F., Mamitsuka, H., Kuma, K.-i., Kanehisa, M. (2004). The Evolutionary Repertoires of the Eukaryotic-Type ABC Transporters in Terms of the Phylogeny of ATP-binding Domains in Eukaryotes and Prokaryotes. Mol Biol Evol 21: 2149-2160 [Abstract] [Full Text]  
• Huang, X., Dong, Y., Zhao, J. (2004). HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. Proc. Natl. Acad. Sci. USA 101: 4848-4853 [Abstract] [Full Text]  
• Liu, D., Golden, J. W. (2002). hetL Overexpression Stimulates Heterocyst Formation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 6873-6881 [Abstract] [Full Text]  
• Meeks, J. C., Elhai, J. (2002). Regulation of Cellular Differentiation in Filamentous Cyanobacteria in Free-Living and Plant-Associated Symbiotic Growth States. Microbiol. Mol. Biol. Rev. 66: 94-121 [Abstract] [Full Text]  
• Herrero, A., Muro-Pastor, A. M., Flores, E. (2001). Nitrogen Control in Cyanobacteria. J. Bacteriol. 183: 411-425 [Full Text]  
• Thiel, T., Pratte, B. (2001). Effect on Heterocyst Differentiation of Nitrogen Fixation in Vegetative Cells of the Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 183: 280-286 [Abstract] [Full Text]  
• Xu, X., Wolk, C. P. (2001). Role for hetC in the Transition to a Nondividing State during Heterocyst Differentiation in Anabaena sp.. J. Bacteriol. 183: 393-396 [Abstract] [Full Text]  
• Hoiczyk, E., Hansel, A. (2000). Cyanobacterial Cell Walls: News from an Unusual Prokaryotic Envelope. J. Bacteriol. 182: 1191-1199 [Full Text]  
• Muro-Pastor, A. M., Valladares, A., Flores, E., Herrero, A. (1999). The hetC Gene Is a Direct Target of the NtcA Transcriptional Regulator in Cyanobacterial Heterocyst Development. J. Bacteriol. 181: 6664-6669 [Abstract] [Full Text]  
• Yoon, H., Golden, J. W. (1998). Heterocyst Pattern Formation Controlled by a Diffusible Peptide. Science 282: 935-938 [Abstract] [Full Text]  
• Zhu, J., Kong, R., Wolk, C. P. (1998). Regulation of hepA of Anabaena sp. Strain PCC 7120 by Elements 5' from the Gene and by hepK. J. Bacteriol. 180: 4233-4242 [Abstract] [Full Text]